Hydrogen sulfide in lipopolysaccharide induced acute lung injury in aged rats.docVIP

 PAGE \* MERGEFORMAT 15 Hydrogen sulfide in lipopolysaccharide induced acute lung injury in aged rats Of: Zhou Xiaohong Huang Xinli Cao Hua Zhang Xuejing LING Yiling [Abstract] Objective To study the hydrogen sulfide (H2S in lipopolysaccharide (LPS induced acute lung injury in aged rats (ALI in rats. Methods 64 SD rats were randomly divided into control group aged, LPS group, NaHS + LPS group, propargyl glycine (PPG + LPS group, 16 rats in each. LPS group, NaHS + LPS group, PPG + LPS group by intratracheal instillation of LPS 200 g / rats rat ALI model by intratracheal group drops Note sterile saline 200 l / only. NaHS + LPS group mold injected intraperitoneally 10 min before NaHS 28 mol / kg, PPG + LPS group mold injected intraperitoneally 10 min prior to PPG 45 mg / kg. or 4 h after administration Animals were sacrificed 8 h, coefficient of determination of the lung observed by light microscopy morphological changes of lung tissue, chemical plasma H2S, NO and CO content in lung tissue malondialdehyde (MDA content of cystathionine lyase (CSE, inducible nitric oxide synthase (iNOS and heme oxygenase 1 (HO 1 activity, detected by immunohistochemistry in lung tissue iNOS, HO 1 protein expression and the absorbance values. intratracheal instillation of LPS results in lung tissue can cause obvious morphological changes of lung MDA content of lung tissue factor and increased plasma H2S concentration and decline in lung tissue CSE activity, lung tissue iNOS activity and expression of iNOS protein, plasma NO content increased lung HO activity and HO 1 protein expression increased plasma CO content increased. pretreated with NaHS significantly reduce LPS induced lung injury, lung index and lung tissue MDA content was decreased, plasma H2S concentration and lung tissue CSE activity increased lung iNOS activity and iNOS protein expression was decreased, plasma NO content decreased , lung HO activity and HO 1 protein expression increased plasma CO levels increased