Hypoxia inducible factor1 in experimental renal interstitial fibrosis in the role of.docVIP

 PAGE \* MERGEFORMAT 16 Hypoxia inducible factor1 in experimental renal interstitial fibrosis in the role of Of: Luo Yanping, Li Jing, Ruijin Bing, Ethnicity population, Wang Xiaoming [Abstract] Objective: To observe the unilateral ureteral obstruction (ulilateral ureteral obstruction, UUO) rat model of renal tissue hypoxia inducible factor 1 @ (HIF 1 @) investigate the expression of HIF 1 @ expression level of tubulointerstitial fibrosis relationship. Methods: 40 female SD rats were randomly divided into 2 groups, sham operation group, UUO group (n = 20). Preparation of UUO rats, day 7, rats were killed 14 days, renal tissue HE and Masson staining, immunohistochemistry and real-time fluorescence quantitative PCR (RT PCR) detection of HIF 1 @, collagen (collagen , Col ) and @ smooth muscle actin (@ smooth muscle actin, @ SMA) in renal tissue expression. Results: The sham operation group at all time points with the UUO group tubulointerstitial fibrosis score, the difference was statistically significant (P lt;0.01). after HIF 1 @ mRNA levels increased with time prolonged obstruction. UUO Group HIF 1 @ protein expression with tubulointerstitial damage increased with increasing, HIF 1 @ protein was mainly distributed in the renal tubules, which express nearly tubule the most obvious. HIF 1 @ expression and tubulointerstitial fibrosis score, @ The expression of SMA and Col levels were positively correlated (R values #8203;#8203;were 0.71,0.68,0.82, P all lt;0.05). Conclusion: HIF 1 @ in the expression of renal tubular epithelial cells may be involved in renal interstitial fibrosis and development process. [Keywords:] Hypoxia inducible factor-1 @; renal interstitial fibrosis; unilateral ureteral obstruction [Abstract] Objective: To investigate the role of hypoxia inducible factor 1 alpha (HIF 1 @) in renal tubulointerstitial lesions induced by unilateral ureteral obstruction (UUO). Methods: Randomly, 40 female SD rats were divided into sham operation g