In several commercially available detergent extraction and detection of hepatitis B virus DNA.docVIP

 PAGE \* MERGEFORMAT 3 In several commercially available detergent extraction and detection of hepatitis B virus DNA Study: Wen-Bin Qin Han Lihong Qin Yanjing Gao Lijun Wang Hailong [Abstract] Objective: To test the effect of several detergents to extract DNA. Methods: 4 solid detergent (secret, white, Diaopai, Tide liquid detergent and 1 (open meters extracted from serum DNA, Polymerase chain reaction (PCR) to detect hepatitis B virus (HBV). Results: 5 detergent can be extracted from the serum HBV DNA, PCR results. Conclusion: This detergent can be used for 5 Extraction of HBV DNA. [Keywords:] detergent; detergent; surface active agent; HBV DNA; PCR Abstract Objective: To examine the effect of extracting DNA with commercial detergent.Methods: Four solid detergents (Aomiao, Baimao, Diaopai, Taizi) and one liquid detergent (Kaimi) were used to extract DNA from serum and HBV was detected with PCR.Results : Five kinds of detergent could be used to extract DNA from serum with all of above five kinds of detergent and HBV could be detected by PCR, with a good result.Conclusion: Above five kinds of detergent can be used to extract HBV DNA. Keywords: Detergent, Cleanser, Surface active agent, HBV DNA, PCR PCR is often used in gene diagnosis technology, it is premised on the extraction of nucleic acids (DNA or RNA. There are many methods of nucleic acid, the reagents used are often related to the surface active agent, such as SDS, Tween 20, TritonX-100, Nonidet-P -40 [1]. surface active agent is a chemical widely used in all areas of human life, including household detergents. detergent surfactants contain more than one purpose is to increase the washing or decontamination effect. detergent can be used to extract DNA has not been reported in this paper a preliminary attempt. 1 Materials and methods 1.1 Materials 4 1.1.1 detergent washing powder: the market to buy, Omo (Unilever Group, or white (Shanghai White Cat Group Limited, Diaopai (NICE G