Medicinal products fingerprint analysis nutmeg_0.docVIP

 PAGE \* MERGEFORMAT 8 Medicinal products fingerprint analysis nutmeg Of: Yuan Zimin, Jia Tianzhu, Zhang Yanqiu, Zu Fengying [Abstract] Objective To establish a product of methanol extract of nutmeg herbs fingerprint analysis method, to study medicine in different parts of the quality of the uniform commercial nutmeg. Methods using high performance liquid chromatography column was ZORBAX EclipseXDB-C18 (4.6 mm × 150 mm, 5 μm), mobile phase: methanol - acetonitrile - water (25:35:40), flow rate 1 mL / min, column temperature: 30 ℃, detection wavelength: 270 nm. to dehydrogenase II isoeugenol to reference. The results establish a fingerprint of medicinal herbs nutmeg, a total of 17 peaks, fingerprint similarity were above 0.9. Conclusion of goods in different parts of commercially available herbs nutmeg no difference, the method is accurate, reliable, and for the meat quality control of medicinal cardamom provide a scientific basis. [Keywords:] nutmeg, dehydrogenase II isoeugenol, fingerprint, high performance liquid chromatography Keywords:: nutmeg, dehydrodiisoeugenol, fingerprint, HPLC Plants for the Nutmeg Nutmeg Nutmeg Myristica fragrans houtt.’s Dried kernel [1]. As a result of origin, storage time is unknown, because quality control standards and low impact, therefore, the quality of the stability of the nutmeg internal medicine, uniform, Controlled research is particularly important. This test uses high performance liquid chromatography (HPLC), nutmeg herbs in different regions for the study of goods, the establishment of the methanol extract fingerprint analysis method, the method is simple, sensitive and accurate, for the meat cardamom quality standard of medicine to provide a scientific basis. 1 Materials 1.1 Instrument Agilent1100 high performance liquid chromatography (chemistation system workstation). 1.2 Reagents Dehydrogenase II isoeugenol reference substance [2], Shenyang Pharmaceutical University, provided by high pe