Medicine two-step sequential treatment on lung tissue of asthmatic mice GATA3 expression and airway inflammation in.doc

 PAGE \* MERGEFORMAT 3 Medicine two-step sequential treatment on lung tissue of asthmatic mice GATA3 expression and airway inflammation in Author: Liu Hsiu-mei Qu Zheng Hai-Shuang Zhou [Abstract] Objective To study the two-step sequential treatment of the traditional Chinese medicine ginseng Schisandra Tonga Xiaoqinglong Decoction on lung tissue of asthmatic mice GATA  3 expression and airway inflammation. Methods 30 SPF grade female mice were randomly divided into normal control group, asthma model group, asthma, stomach feeding model of traditional Chinese medicine group, 10 in each group. To ovalbumin by intraperitoneal injection and inhalation preparation of mouse asthma model, and has to be ginseng Schisandra Tonga Xiaoqinglong Decoction for treatment of asthma in mice. Killed mice in each group after the ELISA, serum protein, dried egg powder (OVA)  IgE levels; lavage fluid (BALF) cells was determined by the number of total number and types of inflammatory cells; HE staining of lung tissue pathology, immunohistochemistry Determination of lung tissue expression levels of GATA  3. The results of gastric feeding medicine asthma model of serum OVA  IgE levels, GATA  3 expression levels were significantly reduced compared with asthma model, higher than the normal control group. Asthma model of gastric feeding Medicines BALF total WBC, percentage of eosinophils and lymphocytes compared with the proportion of asthma model group decreased compared with normal control group increased; while the proportion of phagocytic cells, higher than the asthma model group, lower than the normal control group. Conclusion ginseng Schisandra Tonga Xiao Qing Long Tang may influence the expression of GATA  3 and thus regulate the immune system to control airway inflammation in asthmatic mice, may play an important role in their treatment. [Keywords:] Asthma; mice; GATA  3; ginseng Schisandra decoction; Xiao Qing Long Tang; Asthma / therapy; Animals, laboratory [A