Oleanolic acid on K562 cells in bax bclxL mRNA expression.docVIP

 PAGE \* MERGEFORMAT 8 Oleanolic acid on K562 cells in bax bclxL mRNA expression Of: Zhang Pengxia Xuefang Xi Wang Didier [Abstract] Objective oleanolic acid (OA in human chronic myeloid leukemia K562 cells bcl xL and bax gene expression. Methods OA observed by optical microscope in vitro inhibition of K562 cell proliferation and bcl detected by RT PCR xL and bax mRNA expression. The results with the control group and the DMSO control group, 80 mol / L OA treated cells, cell proliferation was inhibited, OA treatment group were increased bax mRNA, bcl xL mRNA content decreased. Conclusion OA can induce K562 apoptosis, the mechanism of gene expression by upregulating bax and down bcl xL gene expression. [Keywords:] oleanolic acid, K562 cells, bcl 2 family, gene expression At home and abroad in recent years, studies have shown that oleanolic acid (Oleanolic Acid, OA can inhibit apoptosis in HL 60 cells (1), but the OA on the chronic myeloid leukemia cells and its exact mechanism is unclear. In this study, through observation OA of chronic myeloid leukemia K562 cells bcl xL and bax gene expression, K562 cells induced by OA preliminary study apoptosis. 1 Materials and methods 1.1 Drugs and Reagents K562 cells were purchased from Shanghai Institute of cells, RPMI 1640, fetal bovine serum, Hyclone products are, OA reference substances were purchased from Pharmaceutical and Biological Products, molecular formula C30H48O3, molecular weight 456.71, dimethyl sulfoxide (DMSO as the Chinese Academy of Sciences Biomedical Engineering products, DEPC, DNA Marker and RT PCR kit Takara products, penicillin and streptomycin were purchased from Beijing Soledad Technology Co., Ltd.. 1.2 Methods 1.2.1 Drug preparation OA reference substance dissolved in DMSO, the preparation of 80 mmol / L stock solution, -20 storage, use with 10% fetal bovine serum, 1640 preparation of a final concentration of 800 mol / L of the working fluid. 1.2.2 Cell culture an