Olmesartan by AT1 receptors in ventrolateral medulla in spontaneously hypertensive rats decreased angiotensin -induced pressor response to central.doc

 PAGE \* MERGEFORMAT 13 Olmesartan by AT1 receptors in ventrolateral medulla in spontaneously hypertensive rats decreased angiotensin -induced pressor response to central Of: Zhang Shuai Lin Yingzi total winter months Zhu Jie Ren [Abstract] Objective olmesartan in spontaneously hypertensive rats (SHR) of exogenous angiotensin (Ang )-induced pressor response of central and with ventrolateral medulla (RVLM) of vascular 1 angiotensin receptor (AT1) relationship. Methods SHR rats were divided into control group (n = 20, normal drinking water), olmesartan group (n = 20, given olmesartan 30 mg kg-1 d-1) and the WKY group (n = 20, WKY rats, normal drinking water) .4 w, the three groups of rats by intraperitoneal anesthesia, craniotomy positioning RVLM, the femoral venous catheter to observe the injection pressure and heart rate before and after Ang . by reverse transcription polymerase chain reaction (RT PCR) and Western blot methods to detect the RVLM AT1 receptor mRNA and protein in the level, and the optical density. results in the olmesartan group feeding rat tail blood pressure to standard. RVLM After microinjection of Ang three groups of rats mean arterial pressure (MAP) increased to varying degrees, increased rate of olmesartan group MAP was significantly lower than SHR group ((26.3 + -0.75) vs (47.2 + -1.41) mmHg, P lt;0.01), but still higher than the WKY group ((21.5 + -0.72) mmHg, P lt;0.05). three groups of rats heart rate (HR) were increased, but the difference was not statistically significant. olmesartan group of RVLM AT1 receptor mRNA within the average optical density was significantly lower than SHR group ((0.94 + -0.41) vs (2.41 + -0.37), P lt;0.01), but higher than the WKY group (0.81 + -0.22, P lt;0.05). Austria losartan group within the United States RVLM AT1 receptor protein was significantly lower than the average optical density of SHR group ((0.51 + -0.09) vs (0.93 + -0.07), P lt;0.01)), but higher than the WKY group (0.43 + -0.03,