ppGalNAcT2 antisense RNA of gastric cancer cell SGC7901 biological characteristics of.docVIP

 PAGE \* MERGEFORMAT 14 ppGalNAcT2 antisense RNA of gastric cancer cell SGC7901 biological characteristics of Of: JIN Meifang, Shao Xuejun, WU Shi-liang [Abstract] Objective: To study peptides in human gastric cancer cell SGC7901 N acetyl galactosyltransferase 2 (pp GalNAc T2) gene expression and tumor metastasis-related genes MMP2, TGF 1 expression in, as well as on the biological characteristics of cell proliferation and morphological . Methods: The two have built a good anti-sense expression vector was transfected into gastric cancer cells SGC7901, screened by G418, the establishment of a series designed to gastric cancer cell SGC7901 ppGalNAc T2 closed gene expression in sub-cell clones. by confocal microscopy, RT PCR detection of antisense RNA closure pp GalNAc T2 gene expression, the gastric cancer cell SGC7901 of TGF 1, MMP2 expression, cell proliferation and morphological changes. Results: The closure pp GalNAc T2 antisense gene expression, the gastric cancer cell SGC7901 pp GalNAc T2’s significantly lower expression levels, cell morphology changes, proliferation slowed down. The results also show that closed pp GalNAc T2 antisense gene expression could make TGF 1, MMP2 gene expression in the mRNA levels were increased, suggesting that gene expression pp GalNAc T2 may SGC7901 gastric cancer cell invasion and metastasis of an impact. Conclusion: pp GalNAc T2 tumors may be proliferation, invasion and metastasis play a role. [Keywords:] SGC7901 cells, peptide N acetyl galactosyltransferase; matrix metalloproteinase; transforming growth factor 1 [Abstract] Objective: To study the effect on the cell proliferation and the level of MMP2 and TGF 1 in the SGC7901 cells caused by antisense blocking pp GalNAc T2 gene expressionMethods: The antisense expressing vectors of two different length pp GalNAc T2 gene segments were constructed and transfected into SGC7901 cells.A series of subcellular clones aiming at blocking of pp GalNAc T2 gene expression of