Recombinant anti-CD20 scFv-CD80-CD28-ζ non-replicating retroviral Construction.docVIP

 PAGE \* MERGEFORMAT 13 Recombinant anti-CD20 scFv/CD80/CD28/ζ non-replicating retroviral Construction [Abstract] Objective: To construct pLNCX/anti-CD20 scFv/CD80/CD28/ζ recombinant eukaryotic expression vector transfected cell line PA 317, packaging and preparation of recombinant non-replicating retrovirus. Methods: DNA recombinant technology to pBULLET on the CD28-ζ cDNA inserted into the anti-CD20 scFv/CD80 already included in the eukaryotic retroviral vector pLNCX plasmid transfected PA 317 cell lines by G418 selection, the supernatant was harvested Non-replicating retrovirus, the