Rotavirus VP6 gene splicing a new synthesis and sequencing.docVIP

 PAGE \* MERGEFORMAT 8 Rotavirus VP6 gene splicing a new synthesis and sequencing Author: Cai-Hong Zhang, Qiang-Zhe Zhang, Yan-Ming Zhang [Keywords:] rotavirus;, VP6 gene; gene synthesis;, genetic engineering vaccine [Abstract] AIM: To design and obtain novel rotavirus (RV) VP6 gene. METHODS: On the basis of our previous reports, RV VP6 gene was optimized through the gradual splicing method, according to the improved PCR strategy. RESULTS: The optimized cDNA sequence of RV VP6 gene was successfully synthesized, which was about 1220 bp and reached 55% in (GC) content. CONCLUSION: The optimized RV VP6 gene was obviously increased about 20% in (GC) content compared to wildtype RV VP6 gene. This study laid a further strong foundation for the development of novel oral rotavirus genetic engineering vaccine. [Keywords] rotavirus; VP6 gene; genes, synthetic; genetic engineering vaccine [Abstract] Objective: To design was rotavirus (RV) of new VP6 gene. Methods: In this study, the preliminary work, based on the optimization of RV VP6 gene synthesis methods are explored in research. We have applied modified nested-PCR strategy for , through the progressive RV VP6 gene splicing method has been optimized transformation. Results: The successful synthesis of an optimized RV VP6 gene cDNA sequence, the sequence length of 1220 bp, (GC) content of up to 55%. Conclusion: Compared with wild-type RV VP6 gene compared to optimize the synthesis of RV VP6 gene (GC) content increased by about 20%, of this work for the new genetically engineered vaccines effective oral RV-depth study of the foundation. [Keywords:] Rotavirus; VP6 gene; gene synthesis; genetically engineered vaccine 0 Introduction Rotavirus (rotavirus, RV) is a cause of severe diarrhea in infants around the world an important pathogen, RV serious harm and no effective treatment [1], the only way to eradicate RV infection is to develop safe and effective vaccine, which has become a global public health goal is a