Rotenone on cultured astrocytes toxicity.docVIP

 PAGE \* MERGEFORMAT 13 Rotenone on cultured astrocytes toxicity Author: Liu Hui, Zhang Xiaojun, Wu Qiang, DONG Zhao-jun [Keywords:] rotenone; star-shaped glial cells; vitality; proliferation Abstract: Objective To observe the rotenone on cultured astrocytes toxicity characteristics. The cells were cultured rat brain astrocytes using glial fibrillary acidic protein (GFAP) were identified by immunofluorescence. Observed star-shaped glial cells exposed to morphology, cell viability was detected by growth curve analysis of rotenone on star-shaped glial cell proliferation, as well as RTPCR detection of gap junction protein (connexin43, CX43) mRNA expression. Results 05μ mol / L rotenone 24h exposure can cause significant morphological changes; 075,10,20 μ mol / L rotenone exposure and at star-shaped glial cell viability significantly decreased lactate dehydrogenase release increased significantly; 05μ mol / L or more rotenone could inhibit the proliferation of glial cells, CX43 mRNA reduced expression. Conclusion of rotenone on cultured astrocytes can produce significant toxic injury, but compared with the neurons, astrocytes on the same dose of rotenone exposure has a greater tolerance. Keywords:: rotenone; star-shaped glial cells; vitality; proliferation Toxic effects of rotenone on primary cultured astroglia in vitro Abstract: Objective To study the toxic effects induced by rotenone on primary cultured astroglia.Methods Rat astroglia were cultured and identified by glial fibrillary acid protein (GFAP). MTT assay and morphological observation were used to evaluate the toxicity of rotenone on astroglia, cell proliferation and the expression of CX43 mRNA were detected by growth curve analysis and RTPCR.Results Cell morphology was changed after being exposed to 05μ mol / L rotenone for 24 hours; the viability of cell cultures was significantly decreased after being treated with 075,10 and 20μ mol / L rotenone; cell proliferation was inhibited and the expressio