RP-HPLC determination of Xihuangcao diterpenoid components in.doc

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2017-05-05 发布于浙江
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RP-HPLC determination of Xihuangcao diterpenoid components in.doc

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RP-HPLC determination of Xihuangcao diterpenoid components in

RP-HPLC determination of Xihuangcao diterpenoid components in Author: Linchao Show Zhu Chen Chenyangjinyan 【Abstract】 Objective To Xihuangcao of diterpenoids method for determination of quality ingredients to create and merchandise analysis, Xihuangcao provide the basis for the development of quality standards. Methods RP  HPLC determination of 6,7  dehydrogenase Reuling ketone and (15S)  12,16  epoxy  11,14  2-hydroxy  8,11,13  abietane  7  one of the content; mobile phase: 0.05% trifluoroacetic acid  acetonitrile (25:75), detection wavelength: 210 nm; theoretical plate number by 6,7  dehydrogenase Reuling one calculation should not be less than 6000, according to (15S)  12,16  epoxy  11,14  2-hydroxy  8,11,13  abietane  7  one calculation should not be less than 5000. The results of 11 batches of test samples 6,7  dehydrogenase Reuling ketone content of the range of 91.5 ~ 182.5 μg * g-1, (15S) -12,16  epoxy  11,14  2-hydroxy  8, 11,13  abietane  7  ketone content of the range of 35.4 ~ 84.2 μg * g-1, the proposed medicine of 6,7 - dehydro Reuling-one and (15S) -12,16  epoxy  11,14 2-hydroxy  8,11,13   abietane  7  ketone content limits were not less than 113.4 μg * g-1, and 52.3 μg * g-1. Conclusion After systematic approach to school inspection, established by the determination method is simple, stable, exclusive, repeatable characteristics, can be used Xihuangcao herbs and products containing the compound preparation Xihuangcao quality control. Keywords: Xihuangcao; 6,7  dehydrogenase Reuling ketone; (15S)  12,16  epoxy  11,14  2-hydroxy -8,11,13  abietane  7  one; content determination; RP-HPLC Abstract: Objective To establish a method for determination of diterpenoids in different batches of the aerial part of Isodon lophanthoides var. Gerardianus. MethodsAn RP  HPLC method was established for the determination of 6,7  Dehydroroyleanone and (15S)  12,16  Epoxy  11,14  dihydroxy  8,11,13  abietatri