Sandalwood terpene synthase gene cloning and sequence analysis.docVIP

 PAGE \* MERGEFORMAT 10 Sandalwood terpene synthase gene cloning and sequence analysis Study: Haitao Wen, Zhao Hongying, Lin Li, Qiu Xianxiu [Abstract] Objective To get involved in cloning the formation of sandalwood essential oil terpene synthase gene. Methods CTAB LiCl extraction of the heartwood of sandalwood has Edgeworthia total RNA, was cloned by RT PCR terpene synthase gene. Results of the cloned a Sandal terpene synthase gene, the gene coding region 1 731 bp, encoding 576 amino acid residues. Conclusion CTAB LiCl method can extract high quality sandalwood heartwood of total RNA, cloned terpene synthases have coding region. [Keywords:] sandalwood; terpene synthase; gene cloning; sequence analysis Abstract: Objective To clone the terpene synthase gene from Santalum album L. which participates in the synthesis of essential oil. Methods CTAB LiCl treatment was used to extract total RNA from the heartwood of Santalum album L. And RT PCR was employed to clone the terpene synthase gene at the same time.Results One terpene synthase gene consisted of 1 731 bp nucleic acid was cloned from Santalum album L., which encodes 576 amino acid residues. Conclusion CTAB LiCl treatment could extract high quality RNA from the heartwood of Santalum album L ., and the cloned terpene synthase gene had the open reading frame. Keywords:: Santalum album L.; terpene synthase; gene cloning; sequence analysis Rare plants of Chinese sandalwood sandalwood sandalwood (Santalum album L.) heartwood of the trunk, the temperature in the row, appetizers analgesic commonly used Chinese medicine [1]. Sandalwood originating in India, Indonesia and other places, South China Botanical Garden in 1962, introduced from Indonesia Tan Planting in the Guangdong region of Hong success [2]. sandalwood in the case of natural growth to be about 10 years to the initial formation of heartwood aroma (commonly known as knot incense), for 30 years or older to medicinal, essential oil of sanda