SD rat costal cartilage cell differentiation in primary culture and to observe the phenomenon.docVIP

 PAGE \* MERGEFORMAT 15 SD rat costal cartilage cell differentiation in primary culture and to observe the phenomenon Of: Yuanya Jiang Xi Mei Fan, high-Ran, Jang Hyuk [Abstract] Objective To establish a rat cartilage cells (RCC) isolation, culture methods, the process of its passage and form extracellular matrix changes. Methods II by trypsin and collagenase digestion step to obtain a combination of mechanical pipetting scattered single RCC, observe the monolayer culture of cells of different morphology algebra RCC; detected by immunocytochemistry in different algebra RCC proteoglycan and type collagen. costal cartilage in rats by two-step digestion results obtained after high purity of the cartilage cells by immunohistochemistry and stained with toluidine blue positive results, while observing the RCC during subculture in the cells of the dedifferentiation phenomenon. Conclusion chondrocytes can be subcultured to obtain amplification, but only for four generations within cells. [Keywords:] rat; cartilage cells; monolayer culture; to differentiate Abstract: Objective To establish the separating and culturing methods for rat costochondral chondrocyte (RCC), and to investigate their morphous changes in the course of cell passage.Methods Under the help of collagenase II and trypsin s digestion, the scattered single RCC was obtained . The observation of morphological changes of RCC cells cultured by monolayer culture in different generations was carried out. And the detection of the expression of collagen II and proteoglycan of RCC cells in different generations by immunocytochemical method was also implemented. Results High purity cartilage cells were obtained from rat costal cartilages after digested by collagenase II and trypsin. It was confirmed by immunohistochemistry and toluidine blue staining. The dedifferentiation of RCC cells was observed when the RCC cells were cultured by monolayer culture.Conclusions RCC cells can be amplified by passage