SDF-1-CXCR4 promote the role of bone marrow stromal cell migration.docVIP

 PAGE \* MERGEFORMAT 16 SDF-1/CXCR4 promote the role of bone marrow stromal cell migration Of: Cao Yang, Lu Gang, Yu Yang, Fan Zhongkai [Abstract] Objective To study the expression of bone marrow stromal cells, CXCR4, SDF-1BMSCs migration, chemotaxis, analysis of laws and mechanisms to promote migration. To improve and enhance the ability of bone marrow stromal cell migration to find a more effective way. For clinical bone marrow stromal cells lay the foundation for wider applications. Methods bone marrow stromal cells in vitro, using immunohistochemical methods on bone marrow stromal cells were detected CXCR4 receptor expression, the use of isolation rooms across the porous experimental method, the SDF-1 on bone marrow stromal cell migration, chemotaxis, and SDF-1 antagonist used to confirm this. CXCR4 results in the generation of bone marrow stromal cells of less than 5%, SDF-1 could promote bone marrow stromal cell migration. Conclusion Although CXCR4 in bone marrow stromal cells little expression, but the migration of bone marrow stromal cells play a crucial role. [Keywords:] bone marrow stromal cells; stromal cell derived factor -1; migration Abstract: Objective To investigate CXCR4 expresstion of bone mesenchymal stroma cells, and the effects of SDF-1/CXCR4 in promoting migration of bone mesenchymal stroma cells and to analyze the mechanism of migration of bone mesenchymal stroma cells, which aims to find more effective ways of improving migration ability. Methods Bone mesenchymal stroma cells of rats were cultured in vitro. Receptor of CXCR4 was detected by immunohistochemistry. Migration of marrow stroma cells were investigated by empirical method of transwell. Results The receptor of CXCR4 on bone mesenchymal stroma cells was less than 5%, and SDF-1 could promote migration of bone mesenchymal stroma cells. Conclusions Although there is a little the receptor of CXCR4 on bone mesenchymal stroma cells, which plays an important role in promotin