Seed protein electrophoresis Andrographis preliminary study.docVIP

 PAGE \* MERGEFORMAT 11 Seed protein electrophoresis Andrographis preliminary study [Abstract] Objective To study the SDSpolyacrylamide gel electrophoresis to identify the feasibility of Andrographis paniculata seeds, the seed for the identification and breeding of Andrographis provide a scientific basis. Methods of polyacrylamide gel electrophoresis, several of Guangdong Province different sources and different collection of Andrographis paniculata seeds of soluble protein electrophoresis. Results paniculata seeds of different origin in the spectrum between the protein electrophoresis pattern type and intensity of bands, the relative content of protein have some differences on the different harvesting Seed protein electrophoresis of the APN protein bands there are some differences in the intensity. Conclusion polyacrylamide gel electrophoresis can be used for identification of Andrographis paniculata seeds. [Keywords:] andrographolide; polyacrylamide gel electrophoresis; seeds; identification Abstract: ObjectiveTo explore the feasibility of seed identification with SDS, and provide scientific basis for breeding of Andrographis paniculata.Methods SDSpolyacrylamide gel electrophoresis technology was adopted for analyzing soluble proteins of several different sources and different collection time in Guangdong.Results Significant differences were observed in spectral pattern, contents of proteins and intensity of bands of Andrographis paniculata from different origins, and in spectral degree between various collection time.Conclusion Polyacrylamide gel electrophoresis technology can be used for seed identification of Andrographis paniculata seeds. Keywords:: Andrographis paniculata; polyacrylamide gel electrophoresis; seed; identification Andrographis Andrographis paniculata (Burm.f.) Nees Acanthaceae plant is, with clearing and detoxifying, cooling swelling and other functions, commonly used in clinical acute dysentery, gastroenteritis, influenza,