Traditional Chinese Medicine of nickel exposure to oxidative damage inhibition.docVIP

 PAGE \* MERGEFORMAT 20 Traditional Chinese Medicine of nickel exposure to oxidative damage inhibition Authors: Lv Xiaoyun, Pu Xiaoli, Chen, Zhu Zhen, Zhao Jianxiong, Wei Hu to 【Abstract】 Objective To study the traditional Chinese medicine Fuzheng fed Decoction (FJD) in rabbit serum of nickel sulfate (NiSO4) exposed human bronchial epithelial cells (16HBE) content and 8  hydroxyl radicals  2  deoxyguanosine triphosphate enzyme ( 8  oxo  dGTPase) in rats. Methods 16HBE cells were cultured in vitro exposure and NiSO4 by NiSO4 Decoction plus Fuzheng serum containing a common treatment, using laser scanning confocal microscope (LSCM) and real-time fluorescence quantitative RT  PCR detection of free radical content and expression of 8  oxo  dGTPase changes. Results NiSO4 (800μmol / L) exposure 16HBE cells, its free radical content and 8  oxo  dGTPase expression were higher than NiSO4 and righting Decoction (10%) handled a total set of cells, P lt;0  05 ~ 0  01 , the relative standard deviation (RSD)gt; 2%. Conclusion 8  oxo  dGTPase could serve as a nickel-exposure to oxidative stress markers; Fu Zheng Jie Du Decoction of Chinese medicine by eliminating free radicals, down 8  oxo  dGTPase expression of the nickel-exposure to oxidative stress has significant inhibitory effect. Keywords: Fuzheng Jiedu Tang The research group for many years carried out in vivo experiments and clinical studies have shown that occupational diseases, traditional Chinese medicine Fuzheng Decoction (FJD) has a good antagonize nickel toxicity 〔1,2〕. In this paper, ways to study the in vitro detoxification decoction of Chinese medicine nickel sulfate (NiSO4) exposed human bronchial epithelial (16HBE) content and intracellular free radicals 8  hydroxy  2  deoxyguanosine triphosphatase (8  oxo  dGTPase) expression the impact of compound mechanism of action designed to bring additional new information. 1 Materials and methods 1 