Transient isotachophoresis chip - accurate calculation of sieve electrophoresis analysis of DNA length of the coupling.docVIP

 PAGE \* MERGEFORMAT 22 Transient isotachophoresis chip - accurate calculation of sieve electrophoresis analysis of DNA length of the coupling Of: Liu, Kun Yue Liang Canton MO Jian Zhou small cotton [Abstract] established based on the relative migration time ratio method, based on pending side of DNA fragments relative to the upper and lower internal standard migration time ratio of the length of the forecast. Experimental results show that, DNA fragments of the relative migration time ratio of the different analytical conditions with good reproducibility. through the creation of relative migration time ratio relative to the corresponding relationship between DNA fragment length formula, to achieve the chip transient isotachophoresis conditions, accurate forecasts of DNA fragment length. The actual sample analysis confirmed that the ratio based on the calculation of the relative migration time simple and reliable method suitable for chip tITP-CGE analysis to determine the exact length of DNA. [Keywords:] chip electrophoresis, isotachophoresis moment, DNA length calculation, migration time, sample concentration Abstract The migration-time in transient isotachophoresis (tITP) separation is affected by sample salinity as the dependence of ITP time on sample-zone conductivity.The sample-to-sample variation of migration-time in microchip tITP-CGE analysis is an undesired factor for precise DNA sizing.In this work, a DNA sizing method that based on relative migration-time proportion (RMP) was developed to eliminate the effect of sample salinity on sizing precision.RMP was defined as the ratio of the migration-time difference between the target fragment and the lower marker to that between the upper marker and the lower marker.The RMP values #8203;#8203;were tested to be reproducible in microchip tITP-CGE separations irrespective of sample salinity.Size of a target DNA was predicted by fitting its RMP value to the equation derived from RMPs of standard DNA