The mobile phase ionic strength on the RP.docVIP

 PAGE \* MERGEFORMAT 28 The mobile phase ionic strength on the RP Author: Liu Hong-Wu Wang Ding Jianying Chou Shilin [Keywords:],, RP Abstract: In the reversed-phase high performance liquid chromatography (RPHPLC) process, the mobile phase ionic strength can significantly affect the dissociation of compounds such as antibiotics, the chromatographic peak shape, because the mobile phase ionic strength is low, the chromatographic packing fixed Phase easy to overload, so that ionization of the compounds showed a typical chromatographic peak shape overload characteristics - peak shape near the triangle. With the increase in sample volume, chromatographic zone of high concentration of the first half of the retention time decreased, but both chromatographic zone end at the same time, chromatographic peak tailing factor and peak width were significantly increased, while the measured object with the separation between the material also will be worse. Select the appropriate buffer salt, and appropriately increase the mobile phase ionic strength of antibiotics can significantly improve chromatographic peak shape, chromatographic peak tailing factor and peak width are decreased, while between the measured object and its related substances are more easily separated . Keywords:: RPHPLC; antibiotics; chromatographic peak shape; mobile phase; ionic strength Influence of the ionic strength of mobile phase on peak shape of antibiotics in RPHPLC ABSTRACT The ionic strength of mobile phase can significantly affect the peak shape of ionogenic compounds such as antibiotics in reversedphase high performance liquid chromatography (RPHPLC). The influence may be attributed to overloading of ionogenic analytes in lower ionic strength mobile phase. In such phase, peak becomes increasingly rightangled triangle in shape with increasing sample load together with increasing peak width and tailing. The retention time of the highconcentration front decreases with increasing sample load,