硒化羧甲基壳聚糖的抗氧化作用研究.docVIP

Bioprocess生物过程, 2011, 1, 22-25 /10.12677/bp.2011.12006 Published Online December 2011 (/journal/bp/) Study on Antioxidant Activity of Selenium Carboxymethyl-Chitosan# Liang Sun , Xianling Qin , Yan Yang , Xiaojing Zhao , Shengdi Fan , Liming Jin 1 1 2 1 1 1* 1 College of Life Science, Dalian Nationalities University, Dalian 2 College of Life Science, Jinan University, Guangzhou College of Marine Life Science, Ocean University of China, Qingdao Email: jlm@ 3 Received: Sep. 17th, 2011; revised: Oct. 14th, 2011; accepted: Nov. 8th, 2011. Abstract: The antioxidant activities in vitro of selenium carboxymethyl-chitosan were investigated by phe- , pyrogallol autoxidation and T-AOC kit. The results showed that in the setting concentration nanthroline-Fe 2+ range, the antioxidant activities increased with the elevation of concentration. The antioxidant activities of se- lenium carboxymethyl-chitosan were better than that of carboxymethyl-chitosan. The clearance rates of hy- droxyl free radical and superoxide anion free radical of 1mg/mL selenium carboxymethyl-chitosan were 42.68% and 31.16%, respectively, and the total antioxidant activity was 0.863 Unit/mL. The results provide references of low toxicity and effective organic selenium products. Keywords: Carboxymethyl-Chitosan; Selenium Carboxymethyl-Chitosan; Antioxidant Activity 硒化羧甲基壳聚糖的抗氧化作用研究# 孙 良 ，覃仙玲 ，杨 艳 ，赵小菁 ，范圣第 ，金黎明 1* 1 2 3 1 1 1 大连民族学院生命科学学院，大连 2 暨南大学生命科学学院，广州 中国海洋大学海洋生命学院，青岛 3 Email: jlm@ 收稿日期：2011年 9月 17日；修回日期：2011年 10月 14日；录用日期：2011年 11月 8日 摘 要：采用邻二氮菲-Fe氧化法、邻苯三酚自氧化法以及总抗氧化能力试剂盒测定硒化羧甲基壳聚 糖的抗氧化能力。结果表明，在实验设置的浓度范围内，硒化羧甲基壳聚糖的抗氧化能力随着浓度的 增加而增加，且硒化羧甲基壳聚糖的抗氧化能力高于羧甲基壳聚糖。1 mg/mL的硒化羧甲基壳聚糖对 羟自由基的清除率为 42.68%，对超氧阴离子自由基的清除率为 31.16%，总抗氧化能力为 0.863单位/mL。 为研究低毒性、有效的有机补硒产品提供了思路。 2+ 关键词：羧甲基壳聚糖；硒化羧甲基壳聚糖；抗氧化性 1. 引言