Antioxidative and Neuroprotective Activities of the Pre-Germinated Brown Rice Extract英文文献资料.docVIP

Food and Nutrition Sciences, 2012, 3, 135-140 135 /10.4236/fns.2012.31020 Published Online January 2012 (http://www.SciRP.org/journal/fns) Antioxidative and Neuroprotective Activities of the Pre-Germinated Brown Rice Extract Rungtip Soi-ampornkul, Sarawut Junnu, Surin Kanyok, Sompong Liammongkolkul, Wanphen Katanyoo, Supranee Umpornsirirat Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Email: sirsm@mahidol.ac.th Received September 23 , 2011; revised November 3 , 2011; accepted November 12 , 2011 rd rd th ABSTRACT We evaluated the effects of pre-germinated brown rice extract (PGBR ex) with enhanced levels of GABA on prolifera- tion and apoptosis of neuronal SK-N-SH cells line. Firstly, we used HPLC methods to study the level of γ-aminobutyric acid (GABA) in all rice extracts. We found that the concentration of GABA in the PGBR ex were 3 and 8 times higher than the GABA concentration in non-germinated brown rice (BR ex) and white rice (WR ex) compared with the stan- dard GABA respectively. Next we study the protective effects of brown rice extract by investigating various methods, we found that the effects of dose-dependent study by treated with PGBR ex, BR ex and WR ex at (0 - 4000 μg/ml). The data from MTT assay showed that the higher concentration of all rice extracts were not induced toxicity to SK-N-SH cells. To test the protective effect by study the viability of SK-N-SH cells. These results showed that PGBR ex and BR ex can protect cells by significantly increase cells survival up to 29.3% ± 0.01% and 13.4% ± 0.07% (p 0.05) but not WR ex comparable with 150 ?M H2O2 alone which caused cells death 56.9% ± 0.02 % (p 0.05), compared with un- treated cells (control). Next study we test the effect of cells apoptotic by ROS assay and