Berberine Prolongs Life Span and Stimulates Locomotor Activity of Drosophila melanogaster英文文献资料.docVIP

American Journal of Plant Sciences, 2012, 3, 1037-1040 1037 /10.4236/ajps.2012.327123 Published Online July 2012 (http://www.SciRP.org/journal/ajps) Berberine Prolongs Life Span and Stimulates Locomotor Activity of Drosophila melanogaster V. V. Navrotskaya , G. Oxenkrug , L. I. Vorobyova , P. Summergrad 1 2 1 2 1 2 V. N. Karazin Kharkiv National University, Kharkiv, Ukraine; Tufts University, Tufts Medical Center, Boston, USA. Email: goxenkrug@ Received June 11 , 2012; revised July 7 , 2012; accepted July 15 , 2012 th th th ABSTRACT Drosophila melanogaster mutants with deficient kynurenine (KYN) formation from tryptophan (TRP) have longer life span than wild type flies. Administration of alpha-methyl-TRP and 5-methyl-TRY, the inhibitors of TRP-KYN metabo- lism, prolonged life span in wild-type flies. Both inhibitors are not available for human use. Berberine, an isoquinoline alkaloid isolated from Berberis aristata, is known as the herb widely used in traditional Chinese and Indian medicine. Berberin is a strong inhibitor of the enzyme catalyzing TRP conversion into KYN. Considering this particular feature we investigated the effect of berberine on life- and health-span in wild-type Drosophila melanogaster. The results of our study showed that Berberine extended mean, median and maximum life span of female flies. Berberine did not af- fect the number of pupae of filial generation and decreased their lethality. Berberine increased locomotor activity (ver- tical climbing). The results of the study suggest that berberine prolongs life- and improves health-span of Drosophila melanogaster. Berberine might be a candidate drug for prevention and treatment of aging and aging-associated medical and ps