Brazilian Propolis Correlation between Chemical Composition and Antimicrobial Activity英文文献资料.docVIP

Advance Access Publication 11 June 2007 eCAM 2008;5(3)317–324 doi:10.1093/ecam/nem058 Original Article BrazilianPropolis:CorrelationBetweenChemicalCompositionand Antimicrobial Activity Kelly Saloma?o1, Paulo Roberto S. Pereira1, Leila C. Campos2, Cintia M. Borba3, Pedro H. Cabello4, Maria Cristina Marcucci5 and Solange L. de Castro1 1Departamento de Ultra-estrutura e Biologia Celular, 2Departamento de Bacteriologia, 3Departamento de Micologia, 4Departamento de Gene′tica, Instituto Oswaldo Cruz, Fundac?a?o Oswaldo Cruz, Rio de Janeiro and 5Po′s-graduac?a?oemFarma′cia,Nu′cleodePo′s-graduac?a?o,PesquisaeExtensa?o,UniversidadeBandeirantedeSa?o Paulo, Sa?o Paulo, Brazil The chemical composition of ethanol extracts from samples of Brazilian propolis (EEPs) determined by HPLC and their activity against Trypanosoma cruzi, Staphylococcus aureus, Streptococcus pneumoniae, Klebisiella pneumoniae, Candida albicans, Sporothrix schenckii and Paracoccidioidesbrasiliensisweredetermined.Basedonthepredominantbotanicalorigininthe region of samples’ collection, the 10 extracts were separated into three groups: A (B. dracunculifoliatAuraucaria spp), B (B. dracunculifolia) and C (Araucaria spp). Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) and of trypomasti- gotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4) and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN). When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliableresults.However,anoverallanalysisrevealedforgroupAanassociationofS.aureus with caffeic acid (CAF) and dicaffeoylquinic acid 3 (CAFQ3), of S. pneumoniae with CAFQ3 and monocaffeoylquinic acid 2 (CAFQ2) and of T. cruzi also with CAFQ3. For group B, a high