Cell membranes composition is defined in ER and their restitution proceed by en bloc fusion of ER generated transport vesicles英文文献资料.docVIP

Vol.2, No.12, 1437-1447 (2010) Health doi:10.4236/health.2010.212214 Cell membranes composition is defined in ER and their restitution proceed by en bloc fusion of ER generated transport vesicles Amalia Slomiany*, Bronislaw L. Slomiany Research Center, University of Medicine and Dentistry of New Jersey, Newark, USA; *Corresponding Author: slomiabr@ Received 28 September 2010; revised 19 October 2010; accepted 25 October 2010 ABSTRACT nents restored [3,10,11]. In the proposed concepts of the vesicular transport, the endoplasmic reticulum (ER) is depicted as a protein synthetic and folding site apart from its role in the vesicular membrane lipid synthesis and membrane assembly that includes insertion of the integral proteins dedicated for the specific vesicle and destination site. In our view, the independent of each other lipid assembly into the membrane and protein translation and the capture of preassembled membranes’ proteins [2-5,12,13] is lacking precision that is critical to retain cell characteristic features [2-4,14]. It is also not plausible that preassembled or recycled vesicular carriers could attain the degree of the reproducible fidelity that is achieved when protein translation, membrane lipid syn- thesis and vesicle formation occur in the synchrony and is confined to a specific segment of ER. Moreover, the lipid microenvironment that is required for the precise function of membrane integral protein can only be re- produced when the process involves simultaneous mem- brane lipids synthesis, protein intercalation and en bloc membrane replacement [14-17]. Our previously published studies of apical transport in gastric mucosal epithelial cells demonstrated synchrony in the assembly of transport vesicles membrane and synthesis of apoprotein (apomucin) cargo [18,19]. Gas- tric mucosal epithelium elaborates enormous quantity of glycoprotein (mucin) and requires jus