Nucleocytoplasmic Shuttling of the TACC Protein Mia1pAlp7p Is Required for Remodeling of Microtubule Arrays during the Cell Cycle 英文参考文献.docVIP

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Nucleocytoplasmic Shuttling of the TACC Protein Mia1pAlp7p Is Required for Remodeling of Microtubule Arrays during the Cell Cycle 英文参考文献.doc

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Nucleocytoplasmic Shuttling of the TACC Protein Mia1pAlp7p Is Required for Remodeling of Microtubule Arrays during the Cell Cycle 英文参考文献

NucleocytoplasmicShuttlingoftheTACCProteinMia1p/ Alp7pIsRequiredforRemodelingofMicrotubuleArrays duringtheCellCycle YuenChyaoLing.,AleksandarVjestica.,SnezhanaOliferenko* TemasekLifeSciencesLaboratory,Singapore,Singapore Abstract Microtubulearraysareremodeledascellsproceedthroughthecellcycle.Itisimportanttounderstandhowremodelingis regulatedintimeandspace.Infissionyeast,theconservedmicrotubuleassociatedTACC/TOGcomplexplaysanimportant roleinorganizingmicrotubulesthroughoutthecellcycle.Hereweshowthatthiscomplexundergoesnucleocytoplasmic shuttling through the nuclear import and export signals located in the TACC protein Mia1p/Alp7p. When the Crm1p- dependentnuclearexportsignalofMia1pisdisabled,Mia1paccumulatesinthenucleuswhileitspartnerproteinAlp14p/ TOG is restricted to the cytoplasm. This leads to defects in assembly of both interphase arrays and the mitotic spindle. ArtificialtargetingofAlp14ptothenucleuspartiallyrescuesthemitoticspindledefectscausedbylackofMia1pnuclear export.Interestingly,thenuclearexportsequenceofMia1pappearstooverlapwiththeAlp14pbindingsite.Wepropose thatintricateregulationofthesubcellulardistributionofTACC/TOGcomplexesdrivesmicrotubulearrayremodelingascells progressthroughthecellcycle. Citation:LingYC,VjesticaA,OliferenkoS(2009)NucleocytoplasmicShuttlingoftheTACCProteinMia1p/Alp7pIsRequiredforRemodelingofMicrotubuleArrays duringtheCellCycle.PLoSONE4(7):e6255.doi:10.1371/journal.pone.0006255 Editor:KevinG.Hardwick,UniversityofEdinburgh,UnitedKingdom ReceivedMarch24,2009;AcceptedJune10,2009;PublishedJuly16,2009 Copyright:?2009Lingetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: This work was carried out with support from the Singapore Millenium Foundation. The funders had no role in study design, data collection and analysis,decisiontopublish,orpreparationofthemanuscript. Compe