Protective Role of Morin, a Flavonoid, against High Glucose Induced Oxidative Stress Mediated Apoptosis in Primary Rat Hepatocytes 英文参考文献.docVIP

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Protective Role of Morin, a Flavonoid, against High Glucose Induced Oxidative Stress Mediated Apoptosis in Primary Rat Hepatocytes 英文参考文献.doc

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Protective Role of Morin, a Flavonoid, against High Glucose Induced Oxidative Stress Mediated Apoptosis in Primary Rat Hepatocytes 英文参考文献

ProtectiveRoleofMorin,aFlavonoid,againstHigh GlucoseInducedOxidativeStressMediatedApoptosisin PrimaryRatHepatocytes RadhikaKapoor,PoonamKakkar* CSIR-IndianInstituteofToxicologyResearch,Lucknow,India Abstract Apoptosisisanearlyeventofliverdamageindiabetesandoxidativestresshasbeenlinkedtoacceleratetheapoptosisin hepatocytes. Therefore, the compounds that can scavenge ROS may confer regulatory effects on high-glucose induced apoptosis.Inthepresentstudy,primaryrathepatocyteswereexposedtohighconcentration(40mM)ofglucose.Atthis concentration decreased cell viability and enhanced ROS generation was observed. Depleted antioxidant status of hepatocytesunderhighglucosestresswasalsoobservedasevidentfromtranscriptionallevelandactivitiesofantioxidant enzymes. Further, mitochondrial depolarisation was accompanied by the loss of mitochondrial integrity and altered expression of Bax and Bcl-2. Increased translocation of apoptotic proteins like AIF (Apoptosis inducing factor) Endo-G (endonuclease-G)fromitsresidentplacemitochondriatonucleuswasalsoobserved.Cyt-cresidingintheinter-membrane space of mitochondria also translocated to cytoplasm. These apoptotic proteins initiated caspase activation, DNA fragmentation, chromatin condensation, increased apoptotic DNA content in glucose treated hepatocytes, suggesting mitochondria mediated apoptotic mode of cell death. Morin, a dietary flavonoid from Psidium guajava was effective in increasing the cell viability and decreasing the ROS level. It maintained mitochondrial integrity, inhibited release of apoptoticproteinsfrommitochondria,preventedDNAfragmentation,chromatincondensationandhypodiploidDNAupon exposuretohighglucose.ThisstudyconfirmsthecapacityofdietaryflavonoidMorininregulatingapoptosisinducedby highglucoseviamitochondrialmediatedpathwaythroughinterventionofoxidativestress. Citation: Kapoor R,KakkarP(2012)Protective RoleofMorin,aFlavonoid, againstHighGlucose InducedOxidativeStressMediated Apoptosis inPrimaryRat Hepatocytes.PL