Resistance loci affecting distinct stages of fungal pathogenesis use of introgression lines for QTL mapping and characterization in the maize - Setosphaeria turcica pathosystem 英文参考文献.docVIP

Chung et al. BMC Plant Biology 2010, 10:103 /1471-2229/10/103 RESEARCH ARTICLE Open Access Resistance loci affecting distinct stages of fungal Research article pathogenesis: use of introgression lines for QTL mapping and characterization in the maize - Setosphaeria turcica pathosystem Chia-Lin Chung1, Joy M Longfellow1, Ellie K Walsh1, Zura Kerdieh3, George Van Esbroeck4, Peter Balint-Kurti5 and Rebecca J Nelson*1,2 Abstract Background: Studies on host-pathogen interactions in a range of pathosystems have revealed an array of mechanisms by which plants reduce the efficiency of pathogenesis. While R-gene mediated resistance confers highly effective defense responses against pathogen invasion, quantitative resistance is associated with intermediate levels of resistance that reduces disease progress. To test the hypothesis that specific loci affect distinct stages of fungal pathogenesis, a set of maize introgression lines was used for mapping and characterization of quantitative trait loci (QTL) conditioning resistance to Setosphaeria turcica, the causal agent of northern leaf blight (NLB). To better understand the nature of quantitative resistance, the identified QTL were further tested for three secondary hypotheses: (1) that disease QTL differ by host developmental stage; (2) that their performance changes across environments; and (3) that they condition broad-spectrum resistance. Results: Among a set of 82 introgression lines, seven lines were confirmed as more resistant or susceptible than B73. Two NLB QTL were validated in BC4F2 segregating populations and advanced introgression lines. These loci, designated qNLB1.02 and qNLB1.06, were investigated in detail by comparing the introgression lines with B73 for a series of macroscopic and microscopic disease components targeting different stages of NLB development. Repeated greenhouse and field trials revealed that qNLB1.06Tx303 (the Tx303 allele at bin 1.06) reduces the efficiency of fungal pen