Soybean Trihelix Transcription Factors GmGT-2A and GmGT-2B Improve Plant Tolerance to Abiotic Stresses in Transgenic Arabidopsis 英文参考文献.docVIP

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Soybean Trihelix Transcription Factors GmGT-2A and GmGT-2B Improve Plant Tolerance to Abiotic Stresses in Transgenic Arabidopsis 英文参考文献.doc

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Soybean Trihelix Transcription Factors GmGT-2A and GmGT-2B Improve Plant Tolerance to Abiotic Stresses in Transgenic Arabidopsis 英文参考文献

SoybeanTrihelixTranscriptionFactorsGmGT-2Aand GmGT-2BImprovePlantTolerancetoAbioticStressesin TransgenicArabidopsis Zong-MingXie1,2,3.,Hong-FengZou1,2.,GangLei1,2,WeiWei1,2,Qi-YunZhou1,2,Can-FangNiu1,2 , YongLiao1,2,Ai-GuoTian1,BiaoMa1,Wan-KeZhang1,Jin-SongZhang1*,Shou-YiChen1* 1NationalKeyLaboratoryofPlantGenomics,PlantGeneResearchCenter,InstituteofGeneticsandDevelopmentalBiology,ChineseAcademyofSciences,Beijing,China, 2GraduateSchoolofChineseAcademyofSciences,Beijing,China,3XinjiangAcademyofAgriculturalandReclamationScience,Shihezi,China Abstract Background: Trihelix transcription factors play important roles in light-regulated responses and other developmental processes.However,theirfunctionsinabioticstressresponsearelargelyunclear.Inthisstudy,weidentifiedtwotrihelix transcription factor genes GmGT-2A and GmGT-2B from soybean and further characterized their roles in abiotic stress tolerance. Findings:Bothgenescanbeinducedbyvariousabioticstresses,andtheencodedproteinswerelocalizedinnuclearregion. In yeast assay, GmGT-2B but not GmGT-2A exhibits ability of transcriptional activation and dimerization. The N-terminal peptideof153residuesinGmGT-2Bwastheminimalactivationdomainandthemiddleregionbetweenthetwotrihelices mediatedthedimerizationoftheGmGT-2B.TransactivationactivityoftheGmGT-2Bwasalsoconfirmedinplantcells.DNA binding analysisusing yeastone-hybrid assayrevealedthat GmGT-2A couldbindtoGT-1bx, GT-2bx, mGT-2bx-2 andD1 whereasGmGT-2Bcouldbindtothelatterthreeelements.OverexpressionoftheGmGT-2AandGmGT-2Bimprovedplant tolerancetosalt,freezinganddroughtstressintransgenicArabidopsisplants.Moreover,GmGT-2B-transgenicplantshad moregreenseedlingscomparedtoCol-0underABAtreatment.Manystress-responsivegeneswerealteredinGmGT-2A -and GmGT-2B-transgenicplants. Conclusion:TheseresultsindicatethatGmGT-2AandGmGT-2Bconferstresstolerancethroughregulationofacommonset ofgenesandspecificsetsofgenes.GmGT-2BalsoaffectsABAsensitivity. Citation: Xie Z-M, Zou H-F,Lei G,Wei W, Zhou Q-Y,et