Sox9-Haploinsufficiency Causes Glucose Intolerance in Mice 英文参考文献.docVIP

下载本文档

6
0
约5万字
约 12页
2017-05-13 发布于上海
举报
版权申诉

Sox9-Haploinsufficiency Causes Glucose Intolerance in Mice 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Sox9-Haploinsufficiency Causes Glucose Intolerance in Mice 英文参考文献

Sox9-HaploinsufficiencyCausesGlucoseIntolerancein Mice ClaireL.Dubois.,HungPingShih.,PhilipA.Seymour,NishaA.Patel,JamesM.Behrmann,VictoriaNgo, MaikeSander* DepartmentofPediatricsandCellularMolecularMedicine,UniversityofCaliforniaSanDiego,LaJolla,California,UnitedStatesofAmerica Abstract TheHMGboxtranscriptionfactorSox9playsacriticalroleinprogenitorcellexpansionduringpancreasorganogenesisand isrequiredforproperendocrinecelldevelopmentintheembryo.BasedoninvitrostudiesithasbeensuggestedthatSox9 controls expression of a network of important developmental regulators, including Tcf2/MODY5, Hnf6, and Foxa2, in pancreaticprogenitorcells.Here,wesoughtto:1)determinewhetherSox9regulatesthistranscriptionalnetworkinvivoand 2) investigate whether reduced Sox9 gene dosage leads to impaired glucose homeostasis in adult mice. Employing two geneticmodelsoftemporally-controlledSox9inactivationinpancreaticprogenitorcells,wedemonstratethatcontrarytoin vitrofindings,Sox9isnotrequiredforTcf2,Hnf6,orFoxa2expressioninvivo.Moreover,ouranalysisrevealedanovelrolefor Sox9 in maintaining the expression of Pdx1/MODY4, which is an important transcriptional regulator of beta-cell development. We further show that reduced beta-cell mass in Sox9-haploinsufficient mice leads to glucose intolerance duringadulthood.Sox9-haploinsufficientmicedisplayed50%reducedbeta-cellmassatbirth,whichrecoveredpartiallyviaa compensatory increase in beta-cell proliferation early postnatally. Endocrine islets from mice with reduced Sox9 gene dosageexhibitednormalglucosestimulatedinsulinsecretion.OurfindingsshowSox9playsanimportantroleinendocrine developmentbymaintainingNgn3andPdx1expression.GlucoseintoleranceinSox9-haploinsufficientmicesuggeststhat mutationsinSox9couldplayaroleindiabetesinhumans. Citation: DuboisCL, Shih HP,Seymour PA, Patel NA,Behrmann JM, etal.(2011) Sox9-HaploinsufficiencyCauses Glucose Intolerance inMice. PLoSONE 6(8): e23131.doi:10.1371/journal.pone.0023131 Editor:KathrinMaedler,UniversityofBremen