Tissue culture-induced transpositional activity of mPing is correlated with cytosine methylation in rice 英文参考文献.docVIP

BMC Plant Biology BioMedCentral Research article Open Access Tissue culture-induced transpositional activity of mPing is correlated with cytosine methylation in rice Frédéric Ngezahayo?1,2, Chunming Xu?1, Hongyan Wang1, Lily Jiang1, Jinsong Pang1 and Bao Liu*1 Address: 1Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, PR China and 2Ecole Normale Supérieure, B.P. 6983 Bujumbura, Burundi Email: Frédéric Ngezahayo - ngezafre@yahoo.fr; Chunming Xu - xucm848@; Hongyan Wang - hongyan2003@126.com; Lily Jiang - lily528081@; Jinsong Pang - pangjs542@; Bao Liu* - baoliu@ * Corresponding author ?Equal contributors Published: 15 July 2009 Received: 27 December 2008 Accepted: 15 July 2009 BMC Plant Biology 2009, 9:91 doi:10.1186/1471-2229-9-91 This article is available from: /1471-2229/9/91 ? 2009 Ngezahayo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: mPing is an endogenous MITE in the rice genome, which is quiescent under normal conditions but can be induced towards mobilization under various stresses. The cellular mechanism responsible for modulating the activity of mPing remains unknown. Cytosine methylation is a major epigenetic modification in most eukaryotes, and the primary function of which is to serve as a genome defense system including taming activity of transposable elements (TEs). Given that tissue-culture is capable of inducing both methylation alteration and mPing transposition in certain rice genotypes, it provides a tractable system to investigate the possible relationship between the two phenomena. Results: mPing transposition and cytosine met