戊四氮点燃腺苷A1受体敲除小鼠多药转运体的表达及其与COX-2的相关性分析.pdfVIP

华 中 科 技 大 学 博 士 学 位 论 文 The study of the expression of multidrug transporters in the PTZ-kindled adenosine A1 receptors knock-out mice and correlation with COX-2 Neurology Department of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. M.D. Candidate: Ping Shan Supervisor: Prof Suiqiang Zhu Abstract Part One The role of adenosine A1 receptors in seizures and histomorphology changes in mice after pentylenetetrazol-kindled epilepsy Objective: To observe the changes in seizures and histomorphology in adenosine A1 receptors knock-out mice after pentylenetetrazol(PTZ)-kindled epilepsy and investigate the role of adenosine A1 receptors in an experimental model of epilepsy. Methods: The animals were divided into wild type (WT) group (n=20) and adenosine A1 receptors knock-out (KO) group(n=20). The mortality rates, kindling rates, mean times to onsets, start times of seizures, durations of seizures, and the behavioral seizure scales of mice subjected to the pentylenetetrazol (PTZ)-induced kindling of seizures in two groups were measured and compared. HE staining and Nissl staining were adopted to observe the histomorphology of cortex and hippocampus of mice in two groups at 24 hours and 30 days post-kindling. Results: The rates of mortality and kindling in KO group were significantly higher than that in WT group (P0.05). The mean times to onsets and start times of seizures in KO group were significantly shorter than that in WT group, while the durations of s