纳豆激酶液体发酵条件的优化-吉林农业大学学报.PDF

DOI :10.13327/j.j jlau.2005.05.022 吉林农业大学学报　2005, 27 (5):569 ～573 Journal of J ilin Agricultural University 薛　健, 臧学丽, 陈　光 , 王　刚, 高俊鹏 (吉林农业大学生物技术学院, 长春 130118) 　:以纳豆枯草芽孢杆菌(Bacillus subitilis natto)为出发菌株, 对其液体发 生产纳豆激酶(Nattokinase, NK) 的培养基组成(碳源、氮源、碳氮比和金属离子组成)和培养条件(温度、pH 值, 发 时间、接种量和装液量)对 产酶量的影响进行了研究。 结果表明:液体发 培养基的最佳碳源为木糖, 浓度为1.5%;最佳氮源为大豆蛋 白胨, 浓度为2.0%;添加无机盐组分为MgSO 0.05%、CaCl 0.02%、K HPO /KH PO 0.1%/0.1%。 发 培养的 4 2 2 4 2 4 最佳温度为37℃, pH 7.0 时有利于菌体产酶, 最适装液量为100 mL/250mL, 接种量以2%为最佳, 最适种龄为 18 ;发 周期为3 d。 通过优化培养基和发 条件, 产酶量达到 1 081 U/mL。 :纳豆枯草芽孢杆菌;纳豆激酶;液体发 :TS201.3　　　:A 　　　:1000-5684 (2005)05-0569-05 Optimization of Liquid Fermentation Conditions of Nattokinase XUE Jian, ZANG Xue-li, CHEN Guang, WANG Gang, GAO Jun-peng (College of B iotechnology , J ilin Agricultura l University , Changchun 130118, China ) Abstract:Liquid fermentation for nattokinase by Bacillus subitilis natto BSN was investigated.T e com- ponents of its culture medium including carbon source, nitrogen source, t e ratio of carbon source to nitro- gen source and ion composition were studied.T e results s owed t at t e fermentation conditions included temperature, initial pH value,fermentation time, inoculation quantity and volume of t e medium.To fer- mentative medium, t e best carbon source was xylose 1.5%, and t e best nitrogen source was soya pep- tone 2.0%.T e components of inorganic salts were MgSO 0.05%, CaCl 0.02%, and K HPO / 4 2 2 4 KH PO 0.1%/0.1%.37 ℃was t e most suitable temperature for t e production of NK.T e strain ad 2 4