17β-雌二醇MIPs薄膜的制备及表征的初步研究.pdfVIP

山地琅应量物警报 30 ( 2) :149 - 154 , 2011 Joumal of Mountain Agriculture and Biology 17β-雌二醇 MIPs 薄膜的制备及表征的初步研究* 朱秋劲1 气张孝刚I ，霍萌1 ，陆宽1 (1.贵州大学生命科学学院，贵州贵阳 550025; 2. 贵州大学食品科学t程研究中心，贵州贵阳 55∞25) 摘 要:分别利用分子印迹技术(MIT) 与团相微萃取、石英晶体微夭乎(QCM) 联用技术探索聚合液在介质表面合 成分子印迹聚合膜的方法，并对其识;lJl)特技进行表征。结果显示:对国相微萃取头分子印迹聚合膜以 TFMAA 为 功能单体，TRIM 为交联剂聚合得到的分子印迹聚合物薄膜对 17β，雌二醇具有一定的识JlI) 能力和耐热效果。以 20 mmo l/L TFMAA 、 5 mmo l/L 17β- 雌二醇、5mmoνL 四正丁基六氟磷航麟支持电解质和30 mmol/L 的 N- 甲基 -1 -甲统基 -2- 硝基乙烯胶乙蜻溶液组成的聚合液，经电聚合可以在全电极表面成膜。 关键词:分子印迹聚合膜:因相微萃取;石英晶体微天乎:特性表征 中图分类号:俑57.1 ;0636 文献标识码:A 文章编号:1∞8 -0457(2011 )02 -0149-06 Preliminary Study on the Preparation of 17 (3-estradiol MIPs membranes and its characteriza- tion ZHU Qiu-jin.2. ZHANG Xiao-gang , HUO Mengl • LU Kuan (1. College of 4，声 Sciences ， Guizhou Universi- ty , Guiyang Guizhou 55∞25 ， China; 2. F.ωd Science Engineering Research Center , Guizhou University, Guiyang Guizhou 55∞25 , China ) Abstract: Using Molecular Imprinting Technology (MIT) ， ωwell as the combination of Solid Phase Extrac- tion (SPE) and the Quartz Crystal Microbalance , method of polymerization liquid of synthesiz-ing the molec- ularly imprinted polymer film on the dielectric surface was explored , and the identification property of the method was characterized in the present work. 币le results showed that the mol时ularly imprinted polymer film had certain capacity of discemment and heat-resistant effect synthesized by the function monomer-T下MAA and the crosslinking agent-TRIM , when it wωused as solid phase extraction column. The polymerization liquid constituted by 20 mmol • L -, TFMAA , 17 自-estradiol ， 5 mmol • L -, Tetrabutylammonium hexafluorophos-