水稻抗坏血酸过氧化物酶基因OsAPX1表达载体构建及功能分析.pdfVIP

过转基因技术整合到水稻基因组中，可以用作植物基因的改良，为提高水稻产 量和培育抗逆水稻新品奠定基础。 关键词：OsAPX1，抗坏血酸过氧化物酶，抗热，抗氧化 II ABSTRACT Rice is the main grain crops in the world. The production and quality of the rice would be seriously influenced along with the aggravating environment day-by-day. The plants are easy to produce and accumulate the active oxygen under the environment coercion. if the active oxygen in the plants was not cleared off in time it would break the balance of the active oxygen metabolic and result in the oxidation damage and doing the harm to the plants. So elimination of the active oxygen from the plants and enhances oxidation resistance ability of the plants are the present research popular domain. Ascorbate peroxidase(APX), is a key enzyme for the plants to eliminate the active oxygen, widely exists in the plants and some algae. APX can eliminate the active oxygen which produces and accumulates in the plants, its function is to protect the plants from the oxidized damage. The APX can catalyse the hydrogen peroxide break down to water so that reduce the oxidation. APX can strengthen the oxidation resistance of the plants, and played an important part role in the oxidative stress reply process. APX is a polygenes family in the plant, and according to its localization in the cell may divide into 5 kinds : Chloroplast stroma APX(sAPX), mitochondria APX, chloroplast thylakoid APX (tAPX), microbody APX(mbAPX) and cytoplast APX(cAPX). As the rice genome sequencing successfully ，we know rice genome team include 8 APX family member. The rice APX family gene may divide into: APX1 and APX2 belong to the cytoplasm, APX3 and APX4 belong to the peroxidation build, APX5 , APX6 , APX7 and APX8 belong to the chloroplast. In this researc