CD40LIg基因修饰骨髓间充质干细胞对异种胰岛移植排斥反应的抑制作用论文.docVIP

　　CD40LIg基因修饰骨髓间充质干细胞对异种胰岛移植排斥反应的抑制作用论文 .freelmunoglobulin (CD40LIg) modified bone marroesenchymal stem cells (MSC) on the rejection of rat islet xenografts. 【Methods】 The islet xenografts model ent. Forty-five modeled diabetic rats ly divided into three groups orphological changes of grafts munohistochemical staining and cytokines al level on day 2 after transplantation. The average level blood glucose of control group (A), MSC group (B), transfected MSC group(C) increased on day 7,20,47, after transplantation respectively. (2) The grafts of group A, B, and C survived for (9.5 ± 3.2), (25.2 ± 5.3), and(53.6 ± 7.5) days, respectively. There are significant deviation of the grafts survival among group A, B and C(F=6.821,P 0.05). The survival of transplantation rats e as the survival of grafts (F=6.821,P 0.05).(3)In group A, Serum IL-2 and TNF-α concentration IL-4 and IL-10 concentration atoxylin-eosin staining of grafts shomatory cell infiltrate and expression of insulin protein at islets in group B and C. The grafts positively stained for CD40LIg in group C. 【Conclusion】 CD40LIg gene modified bone marroesenchymal stem cells could prolong the survival time of islet xenografts and inhibit the rejection of islet xenografts. Key ellitus; bone marroesenchymal stem cells; CD40LIg 动脉粥样硬化(atherosclerosis,AS)是一种慢性心血管疾病,严重威胁人类健康?尽管动脉粥样硬化是一个多因素参与的复杂病理过程,但血液中的单核细胞迁移至内皮下分化为巨噬细胞后吞噬脂质,特别是氧化型低密度脂蛋白(oxidized loicroRNA) 参与调控了心血管系统的多种功能, 其表达异常与多种心血管疾病的发生和发展密切相关2?MicroRNA-155(miR-155)是一个典型的多功能microRNA,已有资料表明,miR-155在免疫反应?炎症?肿瘤发生等过程中发挥了重要的作用3?最近的研究显示miR-155在巨噬细胞泡沫化过程中表达上调4,但其对巨噬细胞泡沫化和动脉粥样硬化形成的作用尚不清楚?本研究探讨了miR-155对THP-1巨噬细胞泡沫化过程的影响及其可能机制,以评估miR-155是否可作为防治动脉粥样硬化的新靶点? 1 材料与方法 1.1 试剂与仪器 人类单核细胞株THP-1细胞(中国科学院上海细胞生物学研究所);RPMI1640 细胞培养基?胎牛血清?青/链双抗(GIBCOBRL公司);佛波酯(PMA)?油红O?Hoechst33258?蛋白酶抑制剂cocktail(Sigma公司);SR-A和CD36抗体(R D公司);HRP标记的驴抗山羊IgG(Santa Cruz公司);l,l’-dioctadecyl-3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate(DiI)荧光探针(Probe公司);其余试剂均为进口或国产分析纯产品? CO2培养箱(美国Precision公司);Settler T