Cr3对盐藻生长的影响及其在细胞中的富集和分布.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.11 3+ Cr 对盐藻生长的影响及其在细胞中的富集和分布 刘安军，张玉红，郑捷，刘娜 （天津科技大学食品工程与生物技术学院，天津 300457） 摘要：本文研究了 Cr3+对盐藻生长和细胞形态的影响，并对其在盐藻中的富集和在盐藻主要大分子化合物中的分布进行了探索 和分析。结果表明，低浓度的 Cr3+促进盐藻的生长，而高浓度的 Cr3+对其生长产生抑制作用，并对盐藻细胞形态产生不良影响。 CrCl ·6H O 浓度为2 mg/L 时，Cr3+对盐藻生长和 Cr3+富集均产生促进作用，其富集量达到779.64 µg/g，富集率达到85.27%。实验结 3 2 果还表明，铬跟盐藻中主要大分子化合物都有结合作用，而且主要与多糖结合。 关键词：盐藻；Cr3+；细胞形态；富集；分布 文章篇号：1673-9078(2010)11-1164-1168 3+ Effect of Cr on the Growth of Dunaliella salina and its Accumulation and Distribution in Cell LIU An-jun, ZHANG Yu-hong, ZHENG Jie, LIU Na (College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China) Abstract: In this research, the effects of Cr3+ on the growth and morphology of Dunaliella salina was studied and the accumulation and distribution of Cr3+ in the macromolecular compounds within D. salina were also investigated. The results showed that low concentration of Cr3+ could promote the growth of D. salina. In contrast, the high concentration of Cr3+ not only inhibited the D. salina growth, but also had detrimental impacts on cell morphology. When the concentration of Cr3+ was 2 mg/L, both the D. salina growth and accumulation of Cr3+ were promoted, where the accumulation quantity and accumulation rate of Cr3+ reached 779.64 µg/g and 85.27%, respectively. It was also demonstrated that Cr3+ mainly combined with macromolecular compounds within D. salina, in particular polysaccharides. Key words: Dunaliella salina, Cr3+; cell morph