响应面法优化白骨壤果实中黄酮类化合物的提取工艺.pdfVIP

现代食品科技 Modern Food Science and Technology 2011, Vol.27, No.2 响应面法优化白骨壤果实中黄酮类化合物的提取工艺 杨维 1 1，3 2 1 1 ，夏杏洲 ，韩维栋 ，谌素华 ，王维民 （1.广东海洋大学食品科技学院,广东湛江 524088）(2.广东海洋大学农学院,广东湛江 524088) (3.华南理工大学轻工与食品学院,广东广州 510641) 摘要：本文研究利用响应曲面法优化白骨壤果实中黄酮类化合物的提取工艺。在单因素试验的基础上，利用中心组合设计 响应面试验、研究了提取温度、提取时间、乙醇浓度和料液比(m/V)对白骨壤果实黄酮类化合物提取率的影响，并建立了回归模 型。结果表明，最佳提取工艺条件为提取温度 54.4 ℃、提取时间 3.45 h、乙醇体积分数 65.68%、料液比 1:21.81(m/V) ，该工艺 条件下白骨壤果实中黄酮类化合物的理论提取率为 8.651%，实测提取率为 8.613%，回归模型预测与实际情况吻合良好。 关键字：白骨壤；黄酮；响应面法 文章篇号：1673-9078(2011)2-191-195 Optimization of Extraction Technology of Flavonoids from Avicennia marina Fruits by Response Surface Methodology 1 1 2 YANG Wei , XIA Xing-zhou , HAN Wei-dong , CHEN Su-hua, WANG Wei-min (1.Food Science and Technology College of Guangdong Ocean University, Zhanjiang 524088, China) (2.Agriculture College of Guangdong Ocean University, Zhanjiang 524088, China) (3.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: Response surface methodology (RSM) was used to optimize the extraction technology of flavonoids from Avicennia Marina fruits in this paper. On the basis of single factor tests, a central composite design (CCD) experiment was conducted and the response surface analysis was employed to investigate the influence of four main factors including temperature, extracting time, concentration of ethanol, ratio of solvent to raw material on the yield of flavonoids，and then a regression model establi