鹿茸多肽对大鼠软骨细胞增殖的影响.pdfVIP

包 头 医 学 院 学 报 2008年第24卷第2期 l14 JOURNAL OF BAOTOU MEDICAL COLLEGE VoL 24 No．2 20(18 鹿茸多肽对大鼠软骨细胞增殖的影响 陈晓东 林建华 (1．巴彦淖尔市医院骨科，内蒙古 巴彦淖尔015000；2．福建医科大学附属第一医院) 摘 要 目的：探讨鹿茸多肽(PAP)对大鼠软骨细胞体外增殖的影响。方法：采用二次酶消化法获得大鼠关节软骨 细胞进行传代培养并对第四代细胞进行药物干预，利用MTr比色、免疫组化检测增殖细胞核抗原(PCNA)、流式细胞术 检测细胞周期等方法来检测各代次大鼠软骨细胞和药物干预后第四代软骨细胞的增殖情况。结果：大鼠软骨细胞随传 代培养，MTr比色显示增殖呈下降趋势，免疫细胞化学检测PCNA表达减少，流式细胞术显示细胞增殖指数下降；PAP可 以抑制这～趋势，促进传代培养的细胞增殖能力。结论：PAP对传代培养的大鼠软骨细胞的增殖能力有明显促进作用。 关键词 鹿茸多肽；大鼠；软骨细胞；增殖 Effect of Pflose Antler Polypeptides on Proliferation of Rat Chondrocyte in Vitro CHEN Xiaodong，LIN Jianhua (Department ofOrthopedics，BayannaoerHospital，Bayannaoer 015000，China) Abstract Objective：The purpose of the present was to investigate the e ct of pilose antler polypeptides(PAP)on the proliferation of rat chondrocyte in vitro．Methods：The rat articular chondrocytes were isolated by enzyme digestion and sub—cul— tivated in seria1．The 4th chondrocyte was interfered with PAP．The chondrocytes of different generations and the 4th medicine in- tefered generation were detected with the methods of proliferating cell nuclear antigen，MTY(methyl thiazolyl tetrazolium)assay for proliferation and Flow cytometry for analyses of cell cycle distribution and PI(proliferation index)．Results：With chondro— cytes passage time increasing，the proliferating ability of chondrocytes descended．MTY assay showed that the proliferating ability of chondrocytes decreased，the expression of PCNA tapered in the experiment of immunohistochemistry，and PI descended in flow cytometry for analyses．PAP can inhibit the tendency and strengthen the proliferating ability of chondrocytes in serial subcuhiva— tion．Conclusion：PAP can si