加标浓度直读法测定茶叶中不同溶解特性的铜.pdfVIP

146 2014, Vo1.35, NO.04 画自融事 ※分析检测 加标浓度直读法测定茶叶中不同溶解特性的铜 高向阳l ，2 ，魏姜勉2 ，张娜I (1.郑州科技学院食品科学与工程学院，河南郑州 450064; 2.河南农业大学食品科学技术学院，河南郑州 45仅泊2) 摘 要:建立测定茶叶中不同溶解特性铜的一种新方法。茶叶样品依次经过水溶、醇溶和微波消解后，用铜离子选 择性电极加标浓度直读法测定。结果表明: pH4.00的总离子强度调节缓冲剂中， 1.00 mollL硝酸御和1.00 mollL氟 化铀溶液的最佳用量均为5.00mL。所用铜电极有良好的能斯特响应，转换系数大于93%。实验条件下，茶叶样品 6 测定的相对标准偏差为2.4% (n=l l)、加标回收率为94.38%~101.229岛，铜离子的检出限为1.20X 10. mollL，与国 家标准方法进行对照，测定的结果令人满意。 关键词:加标浓度直读法:茶叶;铜;溶解特性 Determination of Copper Ions with Different Dissolution Characteristics in Tea by Concentration Direct-Reading Method after Standard Solution Addition 1 2 1 GAO Xiang-yang 气 WEI Jiang-mian , ZHANG Na (1. School of Food Science and Engin巳ering， University for Science and Technology Zhengzhou , Zhengzhou 45∞64， China; 2. College ofFood Science and Technology , Henan Agricultural University , Zhengzhou 45α)()2， China) Abstract: A new method to detect copper ions with different dissolution characteristics in tea was established. After sequential extraction of tea s创nples with water and alkali , the remaining residue was rnicrowave-digested and detected wi由 a copper-selective electrode by directly reading the concentrations of copper ions. Results showed that the optimal amounts of 1.00 mo皿potassium nitr毗and 1.∞mollL sodium fluoride added were both 5.∞mL in total ionic strength adjustrnent buffer (TISAB) at pH 4.00. The conversion factor of the copper ion selective electrode was more than 939毛