宁波市甲1型流感毒株耐烷胺类药物分子流行病学研究.pdfVIP

2240 中国卫生检验杂志2007年l2月第l7卷第l2期 Chinese Journal of Health Laboratory Technology，Dec 2007：Vol 17 No 12 【微生物检测方法】 宁波市甲1型流感毒株耐烷胺类药物分子流行病学研究 刘健毅，胡逢蛟，高红，傅燕，陈科杰 (浙江省宁波市疾病预防控制中心，浙江宁波 315010) [摘要] 目的：掌握宁波流感监测网分离到的甲1型流感毒株耐烷胺类药物的突变情况，了解其HAl基因片段的变异 状况。方法：利用RT—PCR方法扩增M2和HA1基因片段并测序，将结果与GenBank中代表毒株核苷酸进行比较，用计 算机软件分析结果。结果：14株甲1型流感毒株中有10株发生了耐药突变，且都发生在第31位氨基酸上；与疫苗株A／ New Caledonia／20／99相比，02年宁波毒株HA1基因同源性片段为99．1％，05年同源性为97．1％～98．3％，06年同源性 为97．2％～96．3％。结论：宁波甲1型流感毒株已经发生了较高的耐烷胺类药物突变，同时抗原也发生了一定的变异。 [关键词] 流感；HINI亚型；烷胺类药物；抗药性；序列分析 [中图分类号] R373．1 3 [文献标识码] A [文章编号] 1004—8685(2007)12—2240—02 Incidence of adamantane resistance among influenza A(H1 N1)viruses isolated from Ningbo Liu Jian—yi Hu Feng-jiao．Gao Hong．Fu Yah Chen Ke1 (Ningbo Center for Disease Control and Prevention，Ningbo 315010，China) [Abstract] Objective：To study the incidence of adamantine resistance among influenza A(HI N1)viruses isolated from Ning— bo，and to explore the genetic characteristic of the viruses．Methods：Amplify the nucleotide fragment of M2 and HA1 gene by RT — PCR for sequencing．Then，the homogenei~of the sequence between these influenza viruses and the representative strains in GenBank were analyzed．Results：Ten of 14 H1 N1 virus randomly selected from our surveiliance network contained a change at a— mino acid 3 1(serine to asparagine)in the M2 gene known to be correlated with adamantane resistance．The HA1 nucleotide se— quences show homology of 99．1％ with vaccine strain in 2002．97．1％ ～98．3％ in 2005 and 97．2％ ～96．3％ in 2006．Conclusion：The high proportion of influenza A(HI N1)viruses currently circulating in Ningbo demonstrate adamantane re— sistance and showe antigenic drift． [Key words] Influenza；HI N1 subtypes；Adamantine—derive drug；Drug resistance；Sequence analyze 流感病毒是上呼吸道感染的主要病原体之一，常能暴发