非洲堇无根苗的生根方法研究.pdfVIP

安徽农业科学，Journal ofAnhui Agri．Sci．2007，35(35)：11386，11412 责任编辑 张杨林 责任校对 王 森 非洲堇无根苗的生根方法研究 邢朝斌，李占军，李玉彦，石翠娟 (华北煤炭医学院生物科学系，河北唐山o630oo) 摘要 [目的]建立非洲堇无根苗的生根技术体系。[方法]以非洲堇的无根苗为试材，研究组培法、水培法、扦插法和滤纸桥法4种生 根方法对非洲堇生根的影响。[结果]4种生根方法均能诱导非洲堇无根苗生根．水培法产生不定根的平均根长(2．5。m)和平均生根 数(42条)均显著高于其他方法。扦插法产生的不定根直径大、长势旺，移栽后成活率高达99．3％，可以应用于大规模生产。40 ml营养 液的生根数量最大，但植株高度和根的长势均劣于3Oml营养液无根苗，应选择适宜的营养液体积。[结论]以珍珠岩为基质在试管外 扦插非洲堇生根是可行的，具有广阔的应用前景，对于降低非洲堇大批量生产的成本有重要意义。 关键词 非洲堇；不定根；无根苗 中图分类号 Q943．1 文献标识码 A 文章编号 O5l7—6611(2007)35—11386一Ol Study on Rooting Methods of Rootless Seedling in Saintpaulia ionantha XING Zhao-bin et al (Department of Biological Sciences，North China Coal Medical College，Tangshan．Hebei O63O00) Abstract 【Objective]The aim of the research was to set up rooting technology system of the rootless seedlings of Saintpaulia ionantha．【Method】 Wi【h the rootless seedlings of S ionantha as test materials．the effects of 4 kinds of rooting methods as tissue culture method．water culture method，cutting method and filter paper bridge culture method on the rooting of S ionantha were studied、【Result]4 kinds of rooting methods could allinduce the rooting of the rootless seedlings in S ionantha and average root length (2．5 am)and average root number(42 pieces)of adventitious root produced by water culture method were higher than that of other methods．The adventitious root produced by cutting method had great diameter and strong growth vigor and its survival rate after transplanting reached 99-3％．which could be used in mass production．The rooting quantity of rootless seedlings in 40 ml nutrient solution was most，but its plant height and root growth vigor were more inferior than that of the rootless seedlings in 30 ml nutrient solution、Therefore，the appropriate volume of nutrient solution should be chosen．『Conclusion]With perlite as medium，the rooting of S，ionantha by using in vitro cutting was feasible and h