东亚飞蝗气味结合蛋白的生物信息学分析.pdf

33 2 () Vol. 33 o. 2 2010 6 JOUR AL OF A JI G ORMAL U IVERSITY ( atural Science Edition) Jun, 2010 东亚飞蝗气味结合蛋白的生物信息学分析 陆 峥, 张妍妍, 洪 芳,蒋国芳 (, , 210046) [] (L ocus ta m ig ra tor ia man ilensis ) OBP1 ( GenBank FJ2153221/ACI306961), / , . , 148 , 16 1226, ( pI) 499, C H O S ; C 696 1128 184 223 15 ; /. [] , , , [] Q811. 4 [ ] A [] 2010 Bioinform atic Analysis on theLocusta m igratoria m an ilensis OdorantBinding Proteins I Lu Z heng, Z hang Yanyan, Hong Fang, J iang G uo fang ( Jiangsu Key Laboratory forBioresourceT echnology, School of Life Science, anjing ormalUniversity, anjing 210046, China) Abstract: The nucleotide and am ino acid sequences of odorantbinding protein I ( GenBank accession number: FJ2153221/ACI306961) from L ocusta m ig ratoria m an ilensis were analyzed and predicted by the tools of bioinformatics in the follow ing aspects: the composition, equipotentia,l hydrophobicity or hydrophilicity, the signalpeptide, transmem brane topologicalstructure, protein secondary structure, the composition of homologous protein and phylogenetic relation ship. The results showed that the LmigOBP1 gene encoded a 148 am ino acid polypeptidesw ith a standard insect phero mone/odorant binding protein domain, the estmi ated relative molecular weight of 16 1226, the theoretical pI of 499, and the structural formula ofC H O S , which included Protein kinase C phosphorylation sites and Casein ki 696 1128 184 223 15 nase II phosphorylation sites. K ey words:L ocusta m ig rator ia man ilensis, odorant binding protein (OB