陆地棉亚红株突变性状的连锁遗传规律分析.pdfVIP

HEREDITAS (Beijing) 2009 8 , 31(8): 855―858 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2009.00855 陆地棉亚红株突变性状的连锁遗传规律分析 陈旭升, 狄佳春, 马晓杰, 刘新民 , 210014 以具有绿叶、花瓣红心的陆地棉、海岛棉与具有亚红株、无红心的陆地棉种质为试验材料, 通过经典遗 传学的二点测验分析了亚红株突变的连锁遗传规律; 结果显示, 测交群体(E083 ×B026) ×苏9701 、(B026 ×E083) ×苏9701 的平均交换值为1.35%; 测交群体(E083×海7124) ×苏9701 、(海7124×E083) ×苏9701 的平均交换值 为 2.94% 。同时, 用亚红株、无红心、棕絮与绿叶、红心、白絮种质杂交, 进行三点测验, 结果表明: 基因 Rs 在Lc 1 、R2 之间; 基因Rs 和Lc 1 的遗传距离为42.21 cM, Rs 和R2 的遗传距离为 1.68 cM 。当两对基因同时发生 双交换时, 相互干扰小, 符合系数达0.79 。根据已知的基因遗传距离, 整合后绘制了遗传连锁图: Rs 基因位于 基因R2 、Lc 1 以及分子标记NAU2863 、NAU3735 和NAU1048 、BNL2634 之间; 其中Rs 基因两侧的分子标记 NAU3735 和NAU1048 与它的遗传距离分别为0.1 cM 和0.2 cM 。 陆地棉; 亚红株突变; 遗传距离; 连锁图 Analysis on linkage inheritance of sub-red plant mutant from upland cotton CHEN Xu-Sheng, DI Jia-Chun, MA Xiao-Jie, LIU Xin-Min Institute of Industrial Crops , Jiangsu Academy of Agricultural Sciences , Nanjing 210014, China Abstract: Analysis of linkage inheritance of sub-red plant, a natural mutant from upland cotton, was conducted by two-point test using parents Gossypium barbadense Hai7124 and G. hirsutum B026 both with petal spots and green leaves, and G. hirsutum E083 with normal white flowers and, sub-red plants. The average percentage of recombination was 1.35% when calculating test-cross combining populations derived from E083/B026//Su9701 and B026/E083//Su9701. The average percentage of recombination was 2.94% when calculating test-cross combining populations derived from E083/Hai7124// Su9701 and Hai7124/E083//Su9701. Meanwhile, the genetic stocks havi