不同品系的基因敲除小鼠的生存曲线的观察-中国试验动物学报.docVIP

研究报告 TRPV1和ASIC3基因敲除小鼠的寿命观察 石宏1，何伟1，王晓宇1，胡玲1，宿杨帅1，辛娟娟1，杨兆坤1，韩旭2，高洁2，张毅3，景向红1( （1.中国中医科学院针灸研究所，北京 100700；2.山东中医药大学，济南 250355；3.中国中医科学院中药研究所，北京 100700） 【摘要】目的 观察酸敏感离子通道亚基3(ASIC3，又名ACCN3)基因敲除ASIC3-/-小鼠、香草酸瞬时受体亚型I（TRPV1）基因敲除TRPV1-/-小鼠的生存曲线，为进一步繁殖使用该品系小鼠提供参考。方法 选用105只基因敲除小鼠，其中ASIC3-/-鼠44只，TRPV1-/-鼠61只。观察正常喂养两种小鼠500 d以内的生存情况，并绘制生存曲线，进行生存分析。结果 ASIC3-/-小鼠与 TRPV1-/- 小鼠随着时间的延长，生存概率降低，经比较TRPV1-/-小鼠的生存概率优于ASIC3-/-小鼠的生存概率，两种小鼠的生存时间存在统计学差异，（P = 0.004，P0.01），两种小鼠中不同性别之间的生存曲线无显著学差异。结论 TRPV1-/-小鼠的生存概率优于ASIC3-/-小鼠的生存概率。而两种鼠不同性别之间的生存概率则基本相当。 【关键词】ASIC3-/-；TRPV1-/-；生存分析；小鼠；基因敲除 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2014.010.000 Survival analysis of ASIC3-/- and TRPV1-/- mice SHI Hong1, HE Wei1, WANG Xiao-yu1, HU Ling1, SU Yang-shuai1 , XIN Juan-juan1, YANG Zhao-kun1, HAN Xu2, GAO Jie2, ZHANG Yi3 , JING Xiang-hong1 （1. Institute of Acupuncture and Moxibustion China Academy of Chinese Medical Sciences, Beijing 100700, China； 2. Shandong University of Traditional Chinese Medicine, Jinan 250355 3. Insititue of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700） 【Abstract】 Objective To analyze the survival curve of ASIC3-/- and TRPV1-/- mice, and provide a reference for further breeding of these strains of mice. Method Forty-four ASIC3-/- mice and sixty-one TRPV1-/- mice were included in this study. The survival of the mice was observed in normal feeding for 500 days, survival curves were drawn, and their survival was analyzed. Results With the passing of time, the survival probability was decreased in ASIC3-/- mice and TRPV1-/- mice. The survival probability of TRPV1-/- mice was significantly better than that of ASIC3-/- mice (P = 0.004, P0.01). No significant difference was observed between the males and females of the same strain. Conclusions The survival probability of TRPV1-/- mice is better than that of ASIC3-/- mice. There is no significant gender diff