不同时间针刺对雌性恒河猴生殖内分泌影响实验探究.docVIP

不同时间针刺对雌性恒河猴生殖内分泌影响实验探究(1.福建中医学院,福州350003;2.福建省计划生育科学技术研究所) [摘要]方法:采用2×2析因设计,分别观察不同时间针刺对实验动物月经周期前半期外周血FSH,LH,E2,T分泌水平的影响。结果:(1)早晨组、晚上组、早晚组各激素分泌均出现明显抑制,空白组及非穴组则呈现大致正常的分泌状态。(2)各时间针刺组的各种激素分泌峰的上升速度及峰值增值变化与空白组相比,差异均有非常显著性意义(P0.05)。(3)不同时间针刺组组间比较,对FSH,T分泌峰的上升速度及峰值增值变化的影响由强到弱依次为早晨组、早晚组、晚上组;对LH分泌峰的上升速度及峰值增值变化的影响由强到弱依次为早晨组、晚上组、早晚组;对E2分泌峰的上升速度及峰值增值变化的影响由强到弱依次为晚上组、早晚组、早晨组。(4)针刺1天1次与1天2次比较,其作用差异无显著意义。? [主题词]时间因素;促卵泡成熟激素/血液;黄体生成素/血液;雌二醇/血液;睾酮/血液ExperimentalStudyonAcupunctureatDifferentTimeonEffectsofReproductiveHormonesin theFemaleRhesusLiPei,XuWenqian,AnXiaoying,etal.(FujianCollegeofTCM,Fuzhou350003, China)[Abstract]Methods22factorialdesignwasusedand11femalerhesuseswererandomly dividedintoblankgroup,nonacupointgroup,morninggroup,nightgroupandmorningnigh tgroup,andeffectsofacupunctureatdifferenttimesonlevelsofperipheralbloodFSH, LH,E2andTlevelsinfirsthalfstageofmenstrualcycleintheexperimentalanimalwereobser vedrespectively.Results(1)Secretionoftheabovementionedhormoneswasinhibitedsign ificantlyinthemorninggroup,thenightgroupandthemorningnightgroup,andwasroughlyno rmalintheblankgroupandthenonacupointgroup;(2)Thereweresignificantdifferencesi ntheraisingvelocityofthesecretingpeakofthevarioushormonesandthepeakincreaseva luesofthevarioushormonesastheacupuncturegroupscomparedwiththeblankgroup (P0.05);(3)Effectsofacupunctureatdifferenttimesontheraisingvelo cityofFSHandTsecretingpeakandincreaseofpeakvaluewerethemorninggroup,themorning nightgroup,thenightgroupfromstrongtoweakinorder,butforLHsecretingpeakwasthemorn inggroup,thenightgroup,themorningnightgroup;forE2wasthenightgroup,themornin gnightgroupandthemorninggroup;(4)Therewasnosignificantdifferenceintheeffectsbetw eenacupunctureoncedailyandtwicedaily.? [Keywords]TimeFactors;FSH/blood;LH/blood;Estradiol/blood;Testosterone/blood在人类中,各种生理生化功能、行为和反应以至细胞形态和结构等都具有节律变化。本课题组的前期实验已证实[1,2]:根据生殖内分泌周期性变化的规律,在月经周期卵泡期实施恰当的治疗,对促卵泡成熟激素(FSH)、黄体生成素(LH)、雌二醇(E2)、和睾酮(T)的分泌高峰有显著的抑制效应,并认为[3]针灸时效性是影响针