人参二醇组皂苷对LPS致休克大鼠肝脏TLR2、TLR9 mRNA表达的影响.pdfVIP

· l16· 中国病理生理杂志 Chinese Journal of Pathophysiology 2008，24(1)：1 16—1 18 [文章编号] 1000—47l8(2008)Ol-0116—03 人参二醇组皂苷对LPS致休克大鼠肝脏 TLR2、TLR9 mRNA表达的影响 王 志。， 李洪岩。， 吕文伟 ， 刘姗姗 ， 穆桂芳。， 李 扬。， 孙连坤‘△ (吉林大学基础医学院 病理生理学教研室 2药理学教研室 3吉林大学2002级临床医学，吉林长春130021) [摘 要] 目的：观察人参二醇组皂苷(PDS)对TLR2和TLR9 mRNA表达的影响，探讨PDS抗休克的分子生 物学机制。方法：大鼠随机分为对照(contro1)组、LPS休克(LPS)组、人参二醇组皂苷小剂量(LPS+PDSL)组和人 参二醇组皂苷中剂量(LPS+PDSM)组。大鼠舌下静脉注射LPS(4 ms／kg)4 h后测定血清中NOS活性、NO含量， 肝组织中LPO含量、SOD活性以及TLR2、TLR4 mRNA的表达。结果：LPS+PDSL组和LPS+PDSM组NOS活性、 NO含量和LPO含量明显低于LPS组，SOD活性明显高于LPS组(P0．05)；LPS+PDSL组和LPS+PDSM组TLR2 mRNA表达明显低于LPS组，TLR9 mRNA表达无变化。结论：PDS通过下调肝组织中TLR2 mRNA表达，降低NOS 活性、NO含量，对肝脏有保护作用。 [关键词] 脂多糖类；人参；受体，Toll样 [中图分类号] R363 [文献标识码] A Effects of panaxadiol saponin on TLR2 and TLR9 mRNA expression in LPS induced shock rats WANG Zhi ，LI Hong—yan ，曲 Wen—wel～，UU Shan—shan ，MU Gui—fang ，LI Yang ．SUN Lian—kun ( Department ofPathophysiology， Department ofPharmacology，School ofBasic Medical Sciences，朋in University， Grade 2002 ofClinical Medicine Department，JUin University，Changchun 130021，China．E—mail：sunlk@flu．e西 Cn) [ABSTRACT] AIM：To explore the molecular mechanism of panaxadiol saponin(PDS)by observing Toll like re- ceptor(TLR)2 and TLR9 mRNA expression induced by lipopolysaccharide(LPS)．METHODS：Rats were divided into LPS，LPS+PDSL，LPS+PDSM and control group，respectively．Nitric oxide synthase(NOS)activity，nitric oxide(NO) content，LPO content，SOD activity and TLR2 and TLR9 mRNA expression were assayed 4 h after intravenous injection of LPS．RESULTS：NOS activity，NO content，LPO content of LPS+PDSL group and LPS+PDSM group were significantly lower than those in LPS group．TLR2 mRNA expression in the liver tissue ofLPS+PDSL group and LPS+PDSM group Was decreased compared with LPS group．CONCLUSION：PDS has a protective effect on liver t