乙型肝炎e抗原阴、阳性慢性乙型肝炎患者细胞免疫状态与乙型肝炎病毒DNA水平相关分析.pdfVIP

· 166 · 堕压茎茎 堡 旦 旦箜23卷第 — 期 Clinical Focus，February 5，2008，V01 23，No．3 ． 论著 ． 乙型肝炎e抗原阴、阳性慢性乙型肝炎患者 细胞免疫状态与乙型肝炎病毒DNA水平相关分析 吴健林 ，曾志励 ，李国坚 ，吴继周 ，陈茂伟 ，郑 奕 ，张丰晓如 (1．广西医科大学第一附属医院感染性疾病科，广西 南宁530021；2．广西医科大学a．护理学院；b．研究生院，广西南宁530021) 摘 要：目的 探讨乙型肝炎e抗原(HBeAg)阴性及 HBeAg阳性慢性乙型肝炎患者在细胞免疫状态及乙型肝 炎病毒 DNA(HBV DNA)水平之间的相关性。方法 分别应用流式细胞仪、荧光定量聚合酶链反应(PCR)方法对 }王BeAg阴、阳性慢性乙型肝炎患者T淋巴细胞亚群及 HBV DNA进行检测。结果 HBeAg阴性慢性乙型肝炎患者 的HBV DNA水平与CD8一T淋巴细胞比例之间存在正相关，而与CD4／CD8比值之间存在负相关，r值分别为 0．567，一0．601，均P0，01；与总T、CD4 T淋巴细胞比例无相关关系。HBeAg阳性组患者的 HBV DNA水平与 总T、CD4 T、CD81。T淋巴细胞水平相关关系。结论 HBeAg阴性慢性乙型肝炎患者HBV DNA水平与机体细胞 免疫密切相关。 关键词：肝炎，乙型，慢性；T淋巴细胞亚群；DNA，病毒 中图分类号：R512．6 文献标识码：A 文章编号：1004—583X(2008)03—0166-03 Analysis of relationship between cellular immune status and HBV DNA levels in patients with HBeAg-negative and HBeAg-positive chronic hepatitis B WU Jian-lin，ZENG Zhi—li，LI Guo-jian，WU ji—zhou，CHEN Mao—wei，ZHENG Yi，ZHANG Feng—xiao Department of Infectious Diseases，the First Affiliated Hospital of Cruangxi Medical University， Nanning 530021，China ABSTRACT：Objective To investigate the relationship between cellular immune status and HBV DNA levels in the patients with HBeAg-negative and HBeAg-positive chronic hepatitis B．Methods Periphera1 T lymphocyte subsets were examined by flow cytometry，and HBV DNA level was tested by fluorescent quantitative polym erase chain reaction(FQ_PCR)．Results HBV DAN level had a postive correlation with the CD8 T(r一0．567，P0．01)，a negative correlation with the ratio of CD4 ／CD8 T lymphocyte，(r一一0．601，P0．01)，and no relationshipexisted between the total T cell and CD4 T in HBeAg—negative group：however。in HBeAg-positive group there was no correlativity between the HBV DNA level and the he totaI T cel1，CD4 T，CD8 T，and the ratio of CD4／CD8． Conclusion HB