NALP3表达与糖尿病肾病肾间质炎症的关系.docVIP

NALP3表达与糖尿病肾病肾间质炎症的关系 张锦华，张建国，杨聚荣，林利容，詹 俊，李开龙，何娅妮 （400042 重庆，第三军医大学大坪医院野战外科研究所肾内科）摘要 探讨糖尿病肾病iabetic kidney disease, DKD）患者肾组织中NALP3的表达变化肾小管间质炎症的。方法 应用免疫组化检测患者肾小管上皮细胞NALP3及IL-1β的表达变化，ELISA法检测尿液IL-1β水平生化方法检测血清总胆固醇、低密度、24h尿蛋白（问题2：请准确写出检测了哪些指标，不用“等”，与1.1中不一致？）计算出肾小球滤过率(estimated glomerular filtration rate, eGFR)，对各指标与DKDeGFR进行相关性分析。结果 DKD患者IL-1β水平较对照组明显增高（P0.05）肾小管上皮细胞NALP3及IL-1β的表达较对照组显著增高（P0.05）且二者的表达与显著相关（P0.)；激光共聚焦显示DKD患者NALP3IL-1β的增加；NALP3及IL-1β阳性细胞比例与患者年龄、体指数、舒张压24 h尿蛋白、总胆固醇无显著相关性但与血肌酐、血糖、IL-1β、显著相关（P0.0)。结论 DKD患者肾小管上皮细胞NALP3表达与炎性细胞因子IL-1β水平、肾功能改变相关提示NALP3可能DKD肾小管间质炎症反应。 关键词糖尿病肾病；NALP3；IL-1β；炎症基金项目国家自然科学基金 通作者何娅妮，电话：023The role of NALP3 on tubulointerstitial inflammation in diabetic kidney disease Zhang Jinhua, Zhang Jianguo, Yang Jurong, Lin Lirong, Zhan Jun,Li Kailong, HE Yani (Department of Nephrology，Institute of Surgery Research，Daping Hospital，the Third Military Medical University，Chongqing 400042，China) Abstract: Objective To the relationship between renal tissue expresstion of NALP3 and tubulointerstitial inflammatory response in patients with diabetic kidney disease. Methods Thirty patients with diabetic kidney disease were included.The control tissues were pathologically confirmed as normal renal tissues adjacent to the renal angiomyolipoma or kidney?transplant Immunohistochemistry was performed to detecte the expression of renal tubular epithelial cells NALP3 and IL-1β in patients with diabetic kidney disease,serum total cholesterol,LDL cholesterol,SCr and 24h urine protein were measured by biochemical method. Results The expressions of NALP3 and IL-1β in renal proximal tubular epithelial cells both increased dramatically in the diabetic kidney disease patients compared with the control group(P0.05),both of them had positive correlation with Interstitial inflammation(r=0.66, r=0.55, P0.001) Interstitial fibrosis (r=0.65, r=0.50, P0.001)and Tubular atrophy (r=0.63, r=0.56, P0.001), and had negative correlation with eGFR(r=-0.73,