香薷总黄酮超声提取工艺探究.docVIP

香薷总黄酮超声提取工艺探究摘 要：目的：确定香薷中总黄酮的最佳超声提取工艺。方法：采用L【sub】9【/sub】（3【sup】4【/sup】）正交实验，以总黄酮提取得率为考察指标，考察乙醇浓度，提取温度，料液比和超声时间等因素对超声提取工艺的影响，确定其最佳超声提取工艺条件。结果：在所有考察的因素中，料液比和超声时间对香薷中总黄酮超声提取的影响最为显著，最佳提取工艺条件是：加药材质量30倍量的50%乙醇、50℃超声提取30min。结论：超声提取法具有时间短、收率高、耗能小等优点，可用于香薷总黄酮的提取。 关键词：香薷；黄酮；超声提取；工艺研究；正交实验 中图分类号：R284文献标识码：A 文章编号：1673-7717（2011）04-0845-03 Optimization of Ultrasound Extraction of Total Flavonoids from Mosla chinensis Maxim QIAN Ke-chang【sup】1【/sup】, YU Chen-huan【sup】2【/sup】 （1.Chinese Medicine Herbal Tablets Factory, Zhejiang Chinese Medical University, Hangzhou 310053,Zhejiang, China 2.College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China） Abstract:Objective：Ultrasound extraction process of Elavonoids from Mosla chinensis Maxim was studied. Methods：To flavonoid content as the index, with L【sub】9【/sub】（3【sup】4【/sup】） orthogonal experimental ultrasound Ultrasound extraction of total flavonoids was investigated ethanol concentration, extraction temperature, solid to liquid ratio and Ultrasound time and other factors to determine the optimum extraction conditions. Results：In all of the factors, the solid to liquid ratio on total flavonoids Mosla chinensis Maxim influence of Ultrasound extraction was the most significant, and the optimum extraction conditions are: solid to liquid ratio1:30, 50% ethanol, 30 minutes of Ultrasound time, extraction temperature of 50℃. Conclusion：Ultrasonic extraction has a short time, high yield, energy consumption, etc, and can be used for the extraction of total flavonoids from Mosla chinensis Maxim. Key words:Mosla chinensis MaximElavonoidsUltrasound extractionOptimization of Technology；Orthogonal 香薷为唇形科植物石香薷Mosla chinensis Maxim.的地上干燥部分，又名华荠?，主要产于江西、河北、河南等地区，其中以江西产量最大、品质最佳【sup】［1］【/sup】。其味辛，性温，归肺、胃经，具有发汗解表、祛暑化湿、利水消肿之功效，临床主要用于治疗暑湿感冒、恶寒发热、头痛无汗、腹痛吐泻、小便不利等症【sup】［2］【/sup】。现代研究表明，其主要含有挥发油，黄酮类等成分，具有广谱抗菌、抗病毒、抗炎镇痛、免疫调节、抑制中枢神经系统等作用【sup】［3］【/sup】。目前有关香薷挥发油的研究报道较多，但关于非挥发性部位的研究报道较少。本课题组前期研究发现，香薷非挥发性