低强度脉冲超声波联合引导骨组织再生促进牙周骨缺损修复实验探究.docVIP

低强度脉冲超声波联合引导骨组织再生促进牙周骨缺损修复实验探究[摘要] 目的 探讨低强度脉冲超声波（LIPUS）联合引导骨组织再生（GBR）对Beagle犬尖牙牙周骨缺损的修复效应。方法 将8只Beagle犬中每只犬的4颗尖牙随机分配到4组：实验1组（LIPUS处理+GBR+自体骨移植组），实验2组（LIPUS 处理+自体骨移植组），实验3组（GBR+自体骨移植组），空白对照组。在犬尖牙颊侧区根中1/3处制备牙周骨缺损模 型，根据分组，自体骨移植后，实验1组和2组采用LIPUS处理20 min·d-1，超声强度为30 mW·cm-2，实验1、3组植入Bio-Gide胶原膜。在LIPUS处理6、8周后处死Beagle犬，对各组尖牙牙周骨缺损区进行Micro-CT检测和分析。结果 临床观察各组牙周骨缺损面积均不同程度缩小。Micro-CT检测分析表明，各组6周与8周时的骨小梁数目、骨小梁厚度和骨小梁体积均无统计学差异（P＞0.05），而各组之间的骨组织测量指标均有统计学差异（P＜0.05），实验1组新生骨量最多。结论 LIPUS具有促进牙周骨缺损修复的潜能，LIPUS与GBR结合可能更有利于牙周骨缺损的修复与再生。 [关键词] 低强度脉冲超声波； 引导骨组织再生； 骨缺损； 修复效应 [中图分类号] R 782 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.05.009 Low intensity pulsed ultrasound irradiating combined with guided bone regeneration for promoting the repair effect of periodontal bone defect Jiang Xinyi1, Yang Ji2, Chai Zhaowu3, Song Jinlin1, Deng Feng1, Wang Zhibiao4. （1. Dept. of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University; Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China; 2. Dept. of Stomatology, Tongliang County People’s Hospital of Chongqing, Chongqing 402560, China; 3. Dept. of Stomatology, Chongqing Three Gorges Central Hospital, Chongqing 404000, China; 4. Chongqing Key Laboratory of Ultrasound Medical Engineering Co-founded by Chongqing and Ministry of Science and Technology, Chongqing 400016, China） [Abstract] Objective To study the repair effect of low intensity pulsed ultrasound（LIPUS） irradiating combined with guided bone regeneration（GBR） on the defect of Beagle dog canines periodontal bone. Methods Four canine teeth of every beagle dog（8 beagle dogs） were randomly distributed: Group 1（LIPUS disposal + GBR+ autogenous bone graft group）, group 2（LIPUS disposal + autogenous bone graft group）, group 3（GBR + autogenous bone graft group）, blank control group. The model of periodontal bone defect was established in the 1/3 part of the root buccal area. According to the group division, autogenous bone were